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SCAP Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SCAP Antibody (N-term) AP12299a
    SCAP Antibody (N-term) (Cat. #AP12299a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SCAP antibody detected the SCAP protein (arrow).
  • 14 - SCAP Antibody (N-term) AP12299a
    SCAP Antibody (N-term) (Cat. #AP12299a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - SCAP Antibody (N-term) AP12299a
    SCAP Antibody (N-term) (Cat. #AP12299a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession Q12770
Other Accession NP_036367.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 22937
Other Names Sterol regulatory element-binding protein cleavage-activating protein, SCAP, SREBP cleavage-activating protein, SCAP, KIAA0199
Target/Specificity This SCAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human SCAP.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCAP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SCAP
Synonyms KIAA0199
Function Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Research Areas

BACKGROUND

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis.

REFERENCES

Vasseur, F., et al. J. Hum. Genet. 55(4):227-231(2010)
Liu, X., et al. Atherosclerosis 208(2):421-426(2010)
McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009) :
Voora, D., et al. Circ Cardiovasc Genet 1(2):100-106(2008)

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