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PCDH20 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PCDH20 Antibody (Center) AP11655C
    All lanes : Anti-PCDH20 Antibody (Center) at 1:2000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: Rat brain whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PCDH20 Antibody (Center) AP11655C
    All lanes : Anti-PCDH20 Antibody (Center) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PCDH20 Antibody (Center) AP11655C
    PCDH20 Antibody (Center) (Cat. #AP11655c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - PCDH20 Antibody (Center) AP11655C
    Confocal immunofluorescent analysis of PCDH20 Antibody (Center)(Cat#AP11655c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 4 - PCDH20 Antibody (Center) AP11655C
    PCDH20 Antibody (Center) (Cat. #AP11655c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, FC, E
Primary Accession Q8N6Y1
Other Accession NP_073754.2
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 104919 Da
Additional info
Gene ID 64881
Other Names Protocadherin-20, Protocadherin-13, PCDH20, PCDH13
Target/Specificity This PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-472 amino acids from the Central region of human PCDH20.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCDH20 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCDH20
Synonyms PCDH13
Function Potential calcium-dependent cell-adhesion protein.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas

BACKGROUND

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain.

REFERENCES

Suzuki, S.T. Exp. Cell Res. 261(1):13-18(2000)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Cross, S.H., et al. Nat. Genet. 6(3):236-244(1994)

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