DUX4 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Overlay histogram showing U-2 OS cells stained with AP11636c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11636c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Anti-DUX4 Antibody (Center) at 1:2000 dilution + Human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
DUX4 Antibody (Center) (Cat. #AP11636c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DUX4 antibody detected the DUX4 protein (arrow).

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, WB, E
Primary Accession	Q9UBX2
Other Accession	Q6RFH8, P0CJ90, P0CJ89, P0CJ88, P0CJ87, P0CJ86, P0CJ85
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig
Calculated MW	44940 Da

Additional info
Gene ID	100288687
Other Names	Double homeobox protein 4 {ECO:0000312\|HGNC:HGNC:50800}, Double homeobox protein 10 {ECO:0000312\|EMBL:AAK915091}, DUX4 (HGNC:50800), DUX10
Target/Specificity	This DUX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-275 amino acids from the Central region of human DUX4.
Dilution	FC~~1:25 WB~~1:1000
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DUX4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DUX4 (HGNC:50800)
Synonyms	DUX10
Function	Involved in transcriptional regulation. May regulate microRNA (miRNA) expression.
Cellular Location	Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00108, ECO:0000269\|PubMed:15709750, ECO:0000269\|PubMed:21060811}. Note=Actively transported through the nuclear pore complex (NPC)
Tissue Location	Does not seem to be expressed in normal muscle, but is detected in muscle of individuals with FSHD, and also in testis (at protein level) (PubMed:21060811). Does not seem to be expressed in normal muscle, but in muscle of individuals with FSHD, where it may be toxic to cells

Research Areas

DUX4 may be involved in transcriptional regulation.

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Cat# AP11636C

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