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HBB Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - HBB Antibody (C-term) AP11557b
    Overlay histogram showing K562 cells stained with AP11557b (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP12735b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - HBB Antibody (C-term) AP11557b
    All lanes : Anti-HBB Antibody (C-term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: human liver lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - HBB Antibody (C-term) AP11557b
    HBB Antibody (C-term) (Cat. #AP11557b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 14 - HBB Antibody (C-term) AP11557b
    HBB Antibody (C-term) (AP11557b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HBB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P68871
Other Accession P04246, P02101, P02128, P06643, P06642, P02042, P02057, P02112, P02081, P11517, P02089, P02091, P02088, NP_000509.1
Reactivity Human
Predicted Mouse, Rat, Rabbit, Pig, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 3043
Other Names Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, LVV-hemorphin-7, Spinorphin, HBB
Target/Specificity This HBB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-107 amino acids from the C-terminal region of human HBB.
Dilution FC~~1:10~50
WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHBB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HBB
Function Involved in oxygen transport from the lung to the various peripheral tissues. Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
Tissue Location Red blood cells..
Research Areas
The Combination of CRISPR/Cas9 and iPSC Technologies in the Gene Therapy of Human β-thalassemia in Mice.
Author : Ou Z1, Niu X1, He W1, Chen Y1, Song B1, Xian Y1, Fan D1, Tang D2,3, Sun X1.
Sci Rep. 2016 Sep 1;6:32463. doi: 10.1038/srep32463.
27581487
Improved hematopoietic differentiation efficiency of gene-corrected beta-thalassemia induced pluripotent stem cells by CRISPR/Cas9 system.
Author : Song B1, Fan Y, He W, Zhu D, Niu X, Wang D, Ou Z, Luo M, Sun X.
Stem Cells Dev. 2015 May 1;24(9):1053-65. doi: 10.1089/scd.2014.0347. Epub 2015 Feb 5.
25517294

BACKGROUND

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

REFERENCES

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Zhou, D., et al. Nat. Genet. 42(9):742-744(2010)
Onakoya, P.A., et al. Ear Nose Throat J 89(7):306-310(2010)
Belisario, A.R., et al. Acta Haematol. 124(3):162-170(2010)
Prakobkaew, N., et al. Acta Haematol. 124(2):115-119(2010)

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