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PTGS2 Antibody (Center P378)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PTGS2 Antibody (Center P378) AP11553C
    Anti-PTGS2 Antibody (Center P378) at 1:2000 dilution + HACAT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PTGS2 Antibody (Center P378) AP11553C
    PTGS2 Antibody (Center P378) (Cat. #AP11553c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PTGS2 antibody detected the PTGS2 protein (arrow).
  • 14 - PTGS2 Antibody (Center P378) AP11553C
    PTGS2 Antibody (Center P378) (Cat. #AP11553c)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTGS2 Antibody (Center P378) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - PTGS2 Antibody (Center P378) AP11553C
    Confocal immunofluorescent analysis of PTGS2 Antibody (Center P378) (Cat. #AP11553c) with A549 cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession P35354
Other Accession NP_000954.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 5743
Other Names Prostaglandin G/H synthase 2, Cyclooxygenase-2, COX-2, PHS II, Prostaglandin H2 synthase 2, PGH synthase 2, PGHS-2, Prostaglandin-endoperoxide synthase 2, PTGS2, COX2
Target/Specificity This PTGS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 363-391 amino acids from the Central region of human PTGS2.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTGS2 Antibody (Center P378) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTGS2
Synonyms COX2
Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Cellular Location Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein
Research Areas
Predictive value of APAF-1 and COX-2 expression in pathologic complete response to neoadjuvant chemoradiotherapy for patients with locally advanced rectal adenocarcinoma.
Author : Peng H1,2,You K3,4,Zhang R5,Xi S6,Zhang T1,Dong J1,Cai M6,Wang C1,Zhang H6,Zhou T2,Gao Y3,Wen B1.
Oncotarget. 2016 Jun 7;7(23):35233-40. doi: 10.18632/oncotarget.9125.
27153549

BACKGROUND

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

REFERENCES

Duggan, K.C., et al. J. Biol. Chem. 285(45):34950-34959(2010)
Feher, A., et al. Am J Geriatr Psychiatry 18(11):983-987(2010)
Wang, C.H., et al. Anticancer Res. 30(9):3649-3653(2010)
Han, E.H., et al. J. Toxicol. Environ. Health Part A 73 (21-22), 1451-1464 (2010) :
Cao, H., et al. Tohoku J. Exp. Med. 222(1):15-21(2010)

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