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Interferon-inducible protein (IFITM3) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Interferon-inducible protein (IFITM3) Antibody (N-term) AP1153a
    All lanes : Anti-IFITM3 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NCI-H460 whole cell lysate Lane 5: SK-BR-3 whole cell lysate Lane 6: human placenta lysate Lane 7: mouse colon lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Interferon-inducible protein (IFITM3) Antibody (N-term) AP1153a
    Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term)(Cat. #AP1153a).AP1153a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
  • 1 - Interferon-inducible protein (IFITM3) Antibody (N-term) AP1153a
    IFITM3 Antibody (N-term) (Cat. #AP1153a) western blot analysis in Hela,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35ug/lane).This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow).
  • 3 - Interferon-inducible protein (IFITM3) Antibody (N-term) AP1153a
    Fluorescent confocal image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession Q01628
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14632 Da
Additional info
Gene ID 10410
Other Names Interferon-induced transmembrane protein 3, Dispanin subfamily A member 2b, DSPA2b, Interferon-inducible protein 1-8U, IFITM3
Target/Specificity This Interferon-inducible protein (IFITM3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Interferon-inducible protein (IFITM3).
Dilution WB~~1:1000
IF~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsInterferon-inducible protein (IFITM3) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFITM3
Function IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2- mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.
Cellular Location Cell membrane; Single-pass type II membrane protein. Late endosome membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein
Research Areas
The IFITMs Inhibit Zika Virus Replication.
Author : Savidis G1, Perreira JM1, Portmann JM1, Meraner P1, Guo Z2, Green S2, Brass AL3.
Cell Rep. 2016 Jun 2. pii: S2211-1247(16)30687-8. doi: 10.1016/j.celrep.2016.05.074. [Epub ahead of print]
27268505
RIG-I Signaling Is Essential For Influenza B Virus-Induced Rapid Interferon Gene Expression.
Author : Makela SM1, osterlund P2, Westenius V2, Latvala S2, Diamond MS3, Gale M Jr4, Julkunen I5.
J Virol. 2015 Sep 16. pii: JVI.01576-15. [Epub ahead of print]
26378160
The CD225 domain of IFITM3 is required for both IFITM protein association and inhibition of influenza A virus and dengue virus replication.
Author : John SP, Chin CR, Perreira JM, Feeley EM, Aker AM, Savidis G, Smith SE, Elia AE, Everitt AR, Vora M, Pertel T, Elledge SJ, Kellam P, Brass AL.
J Virol. 2013 Jul;87(14):7837-52. doi: 10.1128/JVI.00481-13. Epub 2013 May 8.
23658454
IFITM3 restricts the morbidity and mortality associated with influenza.
Author : Everitt AR, Clare S, Pertel T, John SP, Wash RS, Smith SE, Chin CR, Feeley EM, Sims JS, Adams DJ, Wise HM, Kane L, Goulding D, Digard P, Anttila V, Baillie JK, Walsh TS, Hume DA, Palotie A, Xue Y, Colonna V, Tyler-Smith C, Dunning J, Gordon SB; GenISIS In
Nature. 2012 Mar 25;484(7395):519-23. doi: 10.1038/nature10921.
22446628
IFITM3 inhibits influenza A virus infection by preventing cytosolic entry.
Author : Feeley EM, Sims JS, John SP, Chin CR, Pertel T, Chen LM, Gaiha GD, Ryan BJ, Donis RO, Elledge SJ, Brass AL.
PLoS Pathog. 2011 Oct;7(10):e1002337. doi: 10.1371/journal.ppat.1002337. Epub 2011 Oct 27.
22046135
The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.
Author : Brass AL, Huang IC, Benita Y, John SP, Krishnan MN, Feeley EM, Ryan BJ, Weyer JL, van der Weyden L, Fikrig E, Adams DJ, Xavier RJ, Farzan M, Elledge SJ.
Cell. 2009 Dec 24;139(7):1243-54. doi: 10.1016/j.cell.2009.12.017.
20064371

BACKGROUND

The family of interferon-induced transmembrane protein (Ifitm/mil/fragilis) cell surface proteins may modulate cell adhesion and influence cell differentiation.

REFERENCES

Tanaka,S.S., Dev. Cell 9 (6), 745-756 (2005)

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