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>   首页   >   产品   >   一抗   >   细胞生物学   >   PSMB11 Antibody (C-term)   

PSMB11 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PSMB11 Antibody (C-term) AP11339b
    Anti-PSMB11 Antibody (C-term) at 1:1000 dilution + T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PSMB11 Antibody (C-term) AP11339b
    PSMB11 Antibody (C-term) (Cat. #AP11339b)immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession A5LHX3
Other Accession NP_001093250.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 122706
Other Names Proteasome subunit beta type-11, Proteasome subunit beta-5t, PSMB11
Target/Specificity This PSMB11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human PSMB11.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB11 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB11
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome (By similarity). Plays a pivotal role in development of CD8-positive T cells (By similarity).
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus
Research Areas

BACKGROUND

Proteasomes generate peptides that are presented by major histocompatibility complex (MHC) I molecules to other cells of the immune system. Proteolysis is conducted by 20S proteasomes, complexes of 28 subunits arranged as a cylinder in 4 heteroheptameric rings: alpha-1 to -7, beta-1 to -7, beta-1 to -7, and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM 600307), beta-2 (PSMB7; MIM 604030), and beta-5 (PSMB5; MIM 600306). Three additional subunits, beta-1i (PSMB9; MIM 177045), beta-2i (PSMB10; MIM 176847), and beta-5i (PSMB8; MIM 177046), are induced by gamma-interferon (IFNG; MIM 147570) and are preferentially incorporated into proteasomes to make immunoproteasomes. PSMB11, or beta-5t, is a catalytic subunit expressed exclusively in cortical thymic epithelial cells (Murata et al., 2007 [PubMed 17540904]).

REFERENCES

Tomaru, U., et al. Blood 113(21):5186-5191(2009)
Murata, S., et al. Science 316(5829):1349-1353(2007)

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