- 文献引用 : 3
|Application ||WB, IF, FC, E|
|Other Names||Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2|
|Target/Specificity||This JAK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1101-1132 amino acids from the C-terminal region of human JAK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||JAK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Cytoplasm. Nucleus|
|Tissue Location||Ubiquitously expressed throughout most tissues.|
Author : Feng H1,Chen J1,Wang H1,Cheng Y1,Zou Z1,Zhong Q1,Xu J1.
Lab Invest. 2017 Jun 26. doi: 10.1038/labinvest.2017.59. [Epub ahead of print]
Author : Zhao LC1,2, Li J3, Liao K4, Luo N5, Shi QQ6, Feng ZQ7, Chen DL8.
Int J Mol Sci. 2015 Nov 16;16(11):27411-27421.
Author : Kanda N, Watanabe S.
Endocrinology. 2007 May;148(5):2317-25. Epub 2007 Jan 25.
Provided below are standard protocols that you may find useful for product applications.
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.
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