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KRT25 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - KRT25 Antibody (C-term) AP11252b
    KRT25 Antibody (C-term) (Cat. #AP11252b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KRT25 antibody detected the KRT25 protein (arrow).
  • 4 - KRT25 Antibody (C-term) AP11252b
    KRT25 Antibody (C-term) (Cat. #AP11252b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q7Z3Z0
Other Accession NP_853512.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49318 Da
Additional info
Gene ID 147183
Other Names Keratin, type I cytoskeletal 25, Cytokeratin-25, CK-25, Keratin-25, K25, Keratin-25A, K25A, Type I inner root sheath-specific keratin-K25irs1, KRT25 (HGNC:30839)
Target/Specificity This KRT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human KRT25.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKRT25 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT25 (HGNC:30839)
Function Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs) (By similarity). Plays a role in the cytoskeleton organization (PubMed:26902920).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q8VCW2}.
Tissue Location Strongly expressed in skin and scalp, and weak expression observed in thymus and tongue. In the hair follicle, expressed in Henle layer, Huxley layer and in the inner root sheath cuticle of the hair follicle. Expression extends from the bulb region up to the point of differentiation into the three layers. Also present in the medulla of beard hair (at protein level).
Research Areas

BACKGROUND

This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.

REFERENCES

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Rogers, M.A., et al. Differentiation 72 (9-10), 527-540 (2004) :
Hesse, M., et al. Eur. J. Cell Biol. 83(1):19-26(2004)

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