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>   首页   >   产品   >   一抗   >   发育生物学   >   ECAT1 Antibody (N-term)   

ECAT1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ECAT1 Antibody (N-term) AP11238a
    ECAT1 Antibody (N-term) (Cat. #AP11238a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ECAT1 antibody detected the ECAT1 protein (arrow).
  • 14 - ECAT1 Antibody (N-term) AP11238a
    ECAT1 Antibody (N-term) (Cat. #AP11238a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECAT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ECAT1 Antibody (N-term) AP11238a
    ECAT1 Antibody (N-term) (Cat. #AP11238a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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  • 文献引用 : 2
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q587J8
Other Accession NP_001017361
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24306 Da
Additional info
Gene ID 154288
Other Names KHDC3-like protein, ES cell-associated transcript 1 protein, KHDC3L, C6orf221, ECAT1
Target/Specificity This ECAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the N-terminal region of human ECAT1.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsECAT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KHDC3L
Synonyms C6orf221, ECAT1
Tissue Location Expression appears to be maximal in germinal vesicle oocytes, it tails off through metaphase II oocytes and is undetectable following the completion of the oocyte to embryo transition.
Research Areas
NLRP7 and KHDC3L, the two maternal-effect proteins responsible for recurrent hydatidiform moles, co-localize to the oocyte cytoskeleton.
Author : Akoury E1, Zhang L2, Ao A2, Slim R3.
Hum Reprod. 2015 Jan;30(1):159-69. doi: 10.1093/humrep/deu291. Epub 2014 Oct 29.
25358348
Report of four new patients with protein-truncating mutations in C6orf221/KHDC3L and colocalization with NLRP7.
Author : Reddy R1, Akoury E, Phuong Nguyen NM, Abdul-Rahman OA, Dery C, Gupta N, Daley WP, Ao A, Landolsi H, Ann Fisher R, Touitou I, Slim R.
Eur J Hum Genet. 2013 Sep;21(9):957-64. doi: 10.1038/ejhg.2012.274. Epub 2012 Dec 12.
23232697

REFERENCES

Pierre, A., et al. Genomics 90(5):583-594(2007) Mitsui, K., et al. Cell 113(5):631-642(2003)

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