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|Application ||WB, FC, E|
|Calculated MW||175364 Da|
|Other Names||Dual oxidase 2, 1111-, Large NOX 2, Long NOX 2, NADH/NADPH thyroid oxidase p138-tox, NADPH oxidase/peroxidase DUOX2, NADPH thyroid oxidase 2, Thyroid oxidase 2, p138 thyroid oxidase, DUOX2, LNOX2, THOX2|
|Target/Specificity||This DUOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-542 amino acids from the Central region of human DUOX2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DUOX2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.|
|Cellular Location||Apical cell membrane; Multi- pass membrane protein. Cell junction. Note=Localizes to the apical membrane of epithelial cells. Localizes on internal membrane structures under resting conditions, translocates to the plasma membrane and cell-cell junctions upon challenge with enteric pathogens, such as Escherichia coli|
|Tissue Location||Expressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein level) Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary glands, rectum and testis|
Author : Kirkpatrick CT#1,Wang Y#1,Leiva Juarez MM1,Shivshankar P1,Pantaleón García J2,Plumer AK3,Kulkarni VV1,Ware HH1,Gulraiz F1,Chavez Cavasos MA1,Martinez Zayes G2,Wali S3,Rice AP4,Liu H4,Tour JM5,Sikkema WKA5,Cruz Solbes AS6,Youker KA6,Tuvim MJ1,Dickey BF1,3,
MBio. 2018 May 15;9(3). pii: e00696-18. doi: 10.1128/mBio.00696-18.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX1. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain.
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