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DBH Antibody (N-term P42)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - DBH Antibody (N-term P42) AP11226a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling DBH with AP11226a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 1 - DBH Antibody (N-term P42) AP11226a
    All lanes : Anti-DBH Antibody (N-term P42) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: mouse adrenal gland lysate Lane 3: rat adrenal gland lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, IHC-P, E
Primary Accession P09172
Other Accession NP_000778
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 69065 Da
Additional info
Gene ID 1621
Other Names Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH
Target/Specificity This DBH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human DBH.
Dilution IF~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDBH Antibody (N-term P42) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DBH
Function Conversion of dopamine to noradrenaline.
Cellular Location Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen
Research Areas
Generating trunk neural crest from human pluripotent stem cells.
Author : Huang M1, Miller ML1, McHenry LK1, Zheng T1, Zhen Q1, Ilkhanizadeh S1, Conklin BR2,3, Bronner ME4, Weiss WA1,5.
Sci Rep. 2016 Jan 27;6:19727. doi: 10.1038/srep19727.
26812940

BACKGROUND

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq].

REFERENCES

Fernandez-Castillo, N., et al. Psychiatr. Genet. 20(6):317-320(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Punia, S., et al. Pharmacogenet. Genomics 20(7):435-441(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :

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