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NAT12 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NAT12 Antibody (C-term) AP11188b
    Anti-NAT12 Antibody (C-term) at 1:500 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NAT12 Antibody (C-term) AP11188b
    NAT12 Antibody (C-term) (Cat. #AP11188b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NAT12 antibody detected the NAT12 protein (arrow).
  • 14 - NAT12 Antibody (C-term) AP11188b
    NAT12 Antibody (C-term) (Cat. #AP11188b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NAT12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q147X3
Other Accession Q0IHH1, Q8CES0, NP_001011713.2
Reactivity Human, Mouse
Predicted Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39320 Da
Additional info
Gene ID 122830
Other Names N-alpha-acetyltransferase 30, N-acetyltransferase 12, N-acetyltransferase MAK3 homolog, NatC catalytic subunit, NAA30, C14orf35, MAK3, NAT12
Target/Specificity This NAT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-343 amino acids from the C-terminal region of human NAT12.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNAT12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NAA30
Synonyms C14orf35, MAK3, NAT12
Function Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. Necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate.
Cellular Location Cytoplasm.
Research Areas

REFERENCES

Polevoda, B., et al. BMC Proc 3 SUPPL 6, S2 (2009) :

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