|Application ||FC, WB, E|
|Other Accession||A2A7Q9, NP_001120833.1, NP_699172.2|
|Calculated MW||77925 Da|
|Other Names||E3 ubiquitin-protein ligase RNF19B, 632-, IBR domain-containing protein 3, Natural killer lytic-associated molecule, RING finger protein 19B, RNF19B, IBRDC3, NKLAM|
|Target/Specificity||This RNF19B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-398 amino acids from the Central region of human RNF19B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF19B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1 (PubMed:16709802, PubMed:27485036). Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells (PubMed:10438909). Protects against staurosporin-induced cell death (PubMed:27485036).|
|Cellular Location||Cytoplasmic granule membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Expressed specifically in natural killer cells, activated macrophages and cytotoxic T-cells (PubMed:10438909). Present in natural killer cells (at protein level) (PubMed:10912506). Ubiquitously expressed with high expression in testis (PubMed:27485036)|
Provided below are standard protocols that you may find useful for product applications.
IBRDC3 is a cytolysis-associated transmembrane protein expressed in natural killer (NK) cells and T lymphocytes following cytokine stimulation (Kozlowski et al., 1999 [PubMed 10438909]).
Fortier, J.M., et al. J. Immunol. 176(11):6454-6463(2006)
Kozlowski, M., et al. J. Immunol. 163(4):1775-1785(1999)