|Application ||WB, IHC-P, E|
|Other Accession||B2RZC9, NP_776251.1|
|Calculated MW||14679 Da|
|Other Names||Keratinocyte-associated protein 2, KCP-2, KRTCAP2, KCP2|
|Target/Specificity||This KTAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-162 amino acids from the C-terminal region of human KTAP2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KTAP2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X- Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (PubMed:22467853). May be involved in N-glycosylation of APP (amyloid-beta precursor protein). Can modulate gamma-secretase cleavage of APP by enhancing endoprotelysis of PSEN1 (PubMed:21768116).|
|Cellular Location||Endoplasmic reticulum. Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in skin, heart, placental, liver, skeletal muscle, kidney, pancreas, keratinocytes and dermal fibroblasts.|
Provided below are standard protocols that you may find useful for product applications.
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells.
Shibatani, T., et al. Biochemistry 44(16):5982-5992(2005)
Bonkobara, M., et al. Br. J. Dermatol. 148(4):654-664(2003)