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IFT88 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - IFT88 Antibody (C-term) AP11138b
    IFT88 Antibody (C-term) (Cat. #AP11138b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the IFT88 antibody detected the IFT88 protein (arrow).
  • 1 - IFT88 Antibody (C-term) AP11138b
    IFT88 Antibody (C-term) (Cat. #AP11138b) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the IFT88 antibody detected the IFT88 protein (arrow).
  • 14 - IFT88 Antibody (C-term) AP11138b
    IFT88 Antibody (C-term) (Cat. #AP11138b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IFT88 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - IFT88 Antibody (C-term) AP11138b
    Confocal immunofluorescent analysis of IFT88 Antibody (C-term)(Cat#AP11138b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q13099
Other Accession Q61371, NP_006522.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10
Target/Specificity This IFT88 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 791-820 amino acids from the C-terminal region of human IFT88.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIFT88 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFT88
Synonyms TG737, TTC10
Function Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q61371}. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm {ECO:0000250|UniProtKB:Q61371}. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q61371}. Note=Colocalizes with ENTR1 and gamma-tubulin at the basal body of primary cilia. Colocalizes with ENTR1 and pericentrin at the centrosome
Tissue Location Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas EMBL; U20362; AAA86720.1; -; mRNA EMBL; AK300769; BAG62434.1; -; mRNA EMBL; AL161772; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AL590096; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471075; EAX08272.1; -; Genomic_DNA EMBL; BC030776; AAH30776.2; -; mRNA CCDS; CCDS31944.1; -. [Q13099-1] CCDS; CCDS31945.1; -. [Q13099-2] RefSeq; NP_001305420.1; NM_001318491.1 RefSeq; NP_001305422.1; NM_001318493.1. [Q13099-1] RefSeq; NP_783195.2; NM_175605.4. [Q13099-1] RefSeq; XP_005266610.1; XM_005266553.2 RefSeq; XP_011533543.1; XM_011535241.2 RefSeq; XP_016876247.1; XM_017020758.1. [Q13099-3] UniGene; Hs.187376; - ProteinModelPortal; Q13099; - SMR; Q13099; - BioGrid; 113771; 40 CORUM; Q13099; - IntAct; Q13099; 50 STRING; 9606.ENSP00000323580; - iPTMnet; Q13099; - PhosphoSitePlus; Q13099; - BioMuta; IFT88; - DMDM; 206729873; - EPD; Q13099; - jPOST; Q13099; - MaxQB; Q13099; - PaxDb; Q13099; - PeptideAtlas; Q13099; - PRIDE; Q13099; - ProteomicsDB; 59151; - ProteomicsDB; 59152; -. [Q13099-2] ProteomicsDB; 59153; -. [Q13099-3] DNASU; 8100; - Ensembl; ENST00000319980; ENSP00000323580; ENSG00000032742. [Q13099-1] Ensembl; ENST00000351808; ENSP00000261632; ENSG00000032742. [Q13099-2] GeneID; 8100; - KEGG; hsa:8100; - UCSC; uc001unh.4; human. [Q13099-1] CTD; 8100; - DisGeNET; 8100; - EuPathDB; HostDB:ENSG00000032742.17; - GeneCards; IFT88; - HGNC; HGNC:20606; IFT88 MIM; 600595; gene neXtProt; NX_Q13099; - OpenTargets; ENSG00000032742; - Orphanet; 791; Retinitis pigmentosa PharmGKB; PA134991804; - eggNOG; KOG2003; Eukaryota eggNOG; ENOG410XPPW; LUCA GeneTree; ENSGT00390000015473; - HOGENOM; HOG000258203; - HOVERGEN; HBG059328; - InParanoid; Q13099; - KO; K16474; - OMA; TKNKMFP; - OrthoDB; 259837at2759; - PhylomeDB; Q13099; - TreeFam; TF313218; - Reactome; R-HSA-5610787; Hedgehog 'off' state Reactome; R-HSA-5620924; Intraflagellar transport SIGNOR; Q13099; - ChiTaRS; IFT88; human GeneWiki; IFT88; - GenomeRNAi; 8100; - PRO; PR:Q13099; - Proteomes; UP000005640; Chromosome 13 Bgee; ENSG00000032742; Expressed in 218 organ(s), highest expression level in testis ExpressionAtlas; Q13099; baseline and differential Genevisible; Q13099; HS GO; GO:0005814; C:centriole; IDA:UniProtKB GO; GO:0005813; C:centrosome; IDA:UniProtKB GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB GO; GO:0097546; C:ciliary base; IBA:GO_Central GO; GO:0097542; C:ciliary tip; TAS:Reactome GO; GO:0005929; C:cilium; IDA:UniProtKB GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB GO; GO:0031514; C:motile cilium; ISS:BHF-UCL GO; GO:0097730; C:non-motile cilium; IBA:GO_Central GO; GO:0036126; C:sperm flagellum; ISS:UniProtKB GO; GO:0019894; F:kinesin binding; IBA:GO_Central GO; GO:0060271; P:cilium assembly; ISS:UniProtKB GO; GO:0060122; P:inner ear receptor cell stereocilium organization; IBA:GO_Central GO; GO:0035735; P:intraciliary transport involved in cilium assembly; TAS:Reactome GO; GO:0001822; P:kidney development; IBA:GO_Central GO; GO:1905515; P:non-motile cilium assembly; IBA:GO_Central GO; GO:2000785; P:regulation of autophagosome assembly; ISS:UniProtKB GO; GO:1902017; P:regulation of cilium assembly; ISS:UniProtKB Gene3D; 1.25.40.10; -; 3 InterPro; IPR006597; Sel1-like InterPro; IPR013026; TPR-contain_dom InterPro; IPR011990; TPR-like_helical_dom_sf InterPro; IPR019734; TPR_repeat Pfam; PF13174; TPR_6; 1 Pfam; PF13181; TPR_8; 1 SMART; SM00671; SEL1; 4 SMART; SM00028; TPR; 11 SUPFAM; SSF48452; SSF48452; 1 PROSITE; PS50005; TPR; 9 PROSITE; PS50293; TPR_REGION; 2 1: Evidence at protein level; Alternative splicing; Cell projection; Cilium; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Flagellum; Polymorphism; Reference proteome; Repeat; TPR repeat CHAIN 1 833 Intraflagellar transport protein 88 homolog /FTId=PRO_0000106391 REPEAT 206 239 TPR 1 REPEAT 242 275 TPR 2 REPEAT 281 314 TPR 3 REPEAT 316 347 TPR 4 REPEAT 424 457 TPR 5 REPEAT 459 492 TPR 6 REPEAT 493 526 TPR 7 REPEAT 527 560 TPR 8 REPEAT 561 594 TPR 9 REPEAT 595 628 TPR 10 REPEAT 629 662 TPR 11 REPEAT 663 696 TPR 12 VAR_SEQ 1 9 Missing (in isoform 2) /FTId=VSP_035429 VAR_SEQ 61 79 Missing (in isoform 3) /FTId=VSP_040620 VARIANT 383 383 M -> I (in dbSNP:rs2442455) /FTId=VAR_046464 VARIANT 455 455 S -> N (in dbSNP:rs9509307) /FTId=VAR_046465 VARIANT 671 671 S -> G (in dbSNP:rs9552254) /FTId=VAR_046466 CONFLICT 210 210 F -> S (in Ref. 1; AAA86720) CONFLICT 335 335 K -> E (in Ref. 5; AAH30776) CONFLICT 388 388 I -> V (in Ref. 5; AAH30776) CONFLICT 389 389 M -> T (in Ref. 1; AAA86720) CONFLICT 407 407 Y -> C (in Ref. 1; AAA86720) CONFLICT 608 608 E -> G (in Ref. 5; AAH30776) SEQUENCE 833 AA; 94270 MW; 383887C9D79AF0DA CRC64; MKFTNTKVQM MQNVHLAPET DEDDLYSGYN DYNPIYDIEE LENDAAFQQA VRTSHGRRPP ITAKISSTAV TRPIATGYGS KTSLASSIGR PMTGAIQDGV TRPMTAVRAA GFTKAALRGS AFDPLSQSRG PASPLEAKKK DSPEEKIKQL EKEVNELVEE SCIANSCGDL KLALEKAKDA GRKERVLVRQ REQVTTPENI NLDLTYSVLF NLASQYSVNE MYAEALNTYQ VIVKNKMFSN AGILKMNMGN IYLKQRNYSK AIKFYRMALD QVPSVNKQMR IKIMQNIGVT FIQAGQYSDA INSYEHIMSM APNLKAGYNL TICYFAIGDR EKMKKAFQKL ITVPLEIDED KYISPSDDPH TNLVTEAIKN DHLRQMERER KAMAEKYIMT SAKLIAPVIE TSFAAGYDWC VEVVKASQYV ELANDLEINK AVTYLRQKDY NQAVEILKVL EKKDSRVKSA AATNLSALYY MGKDFAQASS YADIAVNSDR YNPAALTNKG NTVFANGDYE KAAEFYKEAL RNDSSCTEAL YNIGLTYEKL NRLDEALDCF LKLHAILRNS AEVLYQIANI YELMENPSQA IEWLMQVVSV IPTDPQVLSK LGELYDREGD KSQAFQYYYE SYRYFPCNIE VIEWLGAYYI DTQFWEKAIQ YFERASLIQP TQVKWQLMVA SCFRRSGNYQ KALDTYKDTH RKFPENVECL RFLVRLCTDL GLKDAQEYAR KLKRLEKMKE IREQRIKSGR DGSGGSRGKR EGSASGDSGQ NYSASSKGER LSARLRALPG TNEPYESSSN KEIDASYVDP LGPQIERPKT AAKKRIDEDD FADEELGDDL LPE
Research Areas
Basic fibroblast growth factor increases IFT88 expression in chondrocytes.
Author : Zhan D1,Xiang W2,Guo F2,Ma Y1.
Mol Med Rep. 2017 Sep 8. doi: 10.3892/mmr.2017.7449. [Epub ahead of print]
28901443
HDAC6 inhibition suppresses chondrosarcoma by restoring the expression of primary cilia.
Author : Xiang W1,Guo F1,Cheng W2,Zhang J1,Huang J1,Wang R1,Ma Z3,Xu K1.
Oncol Rep. 2017 Jun 2. doi: 10.3892/or.2017.5694. [Epub ahead of print]
28586053

BACKGROUND

This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Robert, A., et al. J. Cell. Sci. 120 (PT 4), 628-637 (2007) :
Khanna, H., et al. J. Biol. Chem. 280(39):33580-33587(2005)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)

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