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HTR2B Antibody (C-term E423)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - HTR2B Antibody (C-term E423) AP11124a
    AP11124a staining HTR2B in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - HTR2B Antibody (C-term E423) AP11124a
    All lanes : Anti-HTR2B Antibody (C-term E423) at 1:2000 dilution Lane 1: human liver lysate Lane 2: human placenta lysate Lane 3: HT-29 whole cell lysate Lane 4: mouse heart lysate Lane 5: mouse spleen lysate Lane 5: rat stomach lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HTR2B Antibody (C-term E423) AP11124a
    HTR2B Antibody (C-term E423) (Cat. #AP11124a) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the HTR2B antibody detected the HTR2B protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P41595
Other Accession NP_000858.3
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54298 Da
Additional info
Gene ID 3357
Other Names 5-hydroxytryptamine receptor 2B, 5-HT-2B, 5-HT2B, Serotonin receptor 2B, HTR2B
Target/Specificity This HTR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-435 amino acids from the C-terminal region of human HTR2B.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHTR2B Antibody (C-term E423) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HTR2B
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down- stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5- hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome
Tissue Location Ubiquitous. Detected in liver, kidney, heart, pulmonary artery, and intestine. Detected at lower levels in blood, placenta and brain, especially in cerebellum, occipital cortex and frontal cortex.
Research Areas
Chronic restraint stress reduces carbon tetrachloride-induced liver fibrosis.
Author : Li M1, Sun Q1, Li S1, Zhai Y1, Wang J1, Chen B1, Lu J1.
Exp Ther Med. 2016 Jun;11(6):2147-2152. Epub 2016 Mar 30.
27284296

BACKGROUND

Multiple receptor subtypes of serotonin neurotransmitters with multiple physiologic functions have been recognized. The 5-HT-2 receptors mediate many of the central and peripheral physiologic functions of serotonin. Cardiovascular effects include contraction of blood vessels and shape changes in platelets; central nervous system effects include neuronal sensitization to tactile stimuli and mediation of hallucinogenic effects of phenylisopropylamine hallucinogens.

REFERENCES

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010) Svejda, B., et al. Cancer 116(12):2902-2912(2010) Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009) Tabakoff, B., et al. BMC Biol. 7, 70 (2009) :

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