注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   免疫学   >   FGL2 Antibody (C-term)   

FGL2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - FGL2 Antibody (C-term) AP11121b
    Overlay histogram showing U-2OS cells stained with AP11121b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11121b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - FGL2 Antibody (C-term) AP11121b
    All lanes : Anti-FGL2 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: KG-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - FGL2 Antibody (C-term) AP11121b
    FGL2 Antibody (C-term) (Cat. #AP11121b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the FGL2 antibody detected the FGL2 protein (arrow).
  • 1 - FGL2 Antibody (C-term) AP11121b
    FGL2 Antibody (C-term) (Cat. #AP11121b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FGL2 antibody detected the FGL2 protein (arrow).
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q14314
Other Accession NP_006673.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50229 Da
Additional info
Gene ID 10875
Other Names Fibroleukin, Fibrinogen-like protein 2, pT49, FGL2
Target/Specificity This FGL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-367 amino acids from the C-terminal region of human FGL2.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGL2
Function May play a role in physiologic lymphocyte functions at mucosal sites.
Cellular Location Secreted.
Tissue Location Constitutively expressed in cytotoxic T-cells.
Research Areas

BACKGROUND

The protein encoded by this gene is a secreted protein that is similar to the beta- and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. This protein may play a role in physiologic functions at mucosal sites.

REFERENCES

Liu, Y., et al. Biochem. Biophys. Res. Commun. 396(2):555-561(2010)
Hsieh, Y.H., et al. Bull. Math. Biol. 72(1):122-132(2010)
Siu, K.L., et al. J. Gen. Virol. 90 (PT 9), 2107-2113 (2009) :
Han, M., et al. J. Biol. Chem. 283(47):32715-32729(2008)
Su, K., et al. World J. Gastroenterol. 14(39):5980-5989(2008)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价