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HPR Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HPR Antibody (Center) AP11111c
    Anti-HPR Antibody (Center) at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HPR Antibody (Center) AP11111c
    Anti-HPR Antibody (Center) at 1:8000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HPR Antibody (Center) AP11111c
    Western blot analysis of lysate from HL-60 cell line, using HPR Antibody (Center)(Cat. #AP11111c). AP11111c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • 14 - HPR Antibody (Center) AP11111c
    HPR Antibody (Center) (Cat. #AP11111c)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HPR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P00739
Other Accession NP_066275.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39030 Da
Additional info
Gene ID 3250
Other Names Haptoglobin-related protein, HPR
Target/Specificity This HPR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the Central region of human HPR.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHPR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HPR
Cellular Location Secreted.
Tissue Location In adult liver the amount of HPR mRNA is at the lower limit of detection, therefore the extent of its expression is at most less than 1000-fold that of the HP1F gene No HPR mRNA can be detected in fetal liver. Expressed in Hep-G2 and leukemia MOLT-4 cell lines.
Research Areas

BACKGROUND

Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression (By similarity).

REFERENCES

Bailey, S.D., et al. Diabetes Care (2010) In press :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Harrington, J.M., et al. J. Biol. Chem. 284(20):13505-13512(2009)
Widener, J., et al. PLoS Pathog. 3(9):1250-1261(2007)
Vanhollebeke, B., et al. Proc. Natl. Acad. Sci. U.S.A. 104(10):4118-4123(2007)

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