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CAD Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - CAD Antibody (Center) AP11110c
    Overlay histogram showing Hela cells stained with AP11110c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11110c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 14 - CAD Antibody (Center) AP11110c
    AP11110c staining CAD in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - CAD Antibody (Center) AP11110c
    Anti-CAD Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 243 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CAD Antibody (Center) AP11110c
    All lanes : Anti-CAD Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 243 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - CAD Antibody (Center) AP11110c
    Overlay histogram showing Hela cells stained with AP11110c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11110c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - CAD Antibody (Center) AP11110c
    All lanes : Anti-CAD Antibody (Center) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 243 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CAD Antibody (Center) AP11110c
    CAD Antibody (Center) (Cat. #AP11110c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow).
  • 14 - CAD Antibody (Center) AP11110c
    CAD Antibody (Center) (Cat. #AP11110c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P27708
Other Accession NP_004332.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 242984 Da
Additional info
Gene ID 790
Other Names CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase, CAD
Target/Specificity This CAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 780-809 amino acids from the Central region of human CAD.
Dilution FC~~1:25
IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAD (HGNC:1424)
Function This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Cellular Location Cytoplasm. Nucleus. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth
Research Areas

BACKGROUND

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.

REFERENCES

Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ahuja, V., et al. J. Inherit. Metab. Dis. 31(4):481-491(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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