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DDX27 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - DDX27 Antibody (C-term) AP11105b
    DDX27 Antibody (C-term) (Cat. #AP11105b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DDX27 antibody detected the DDX27 protein (arrow).
  • 14 - DDX27 Antibody (C-term) AP11105b
    DDX27 Antibody (C-term) (Cat. #AP11105b)immunohistochemistry analysis in formalin fixed and paraffin embedded human prsotate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DDX27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - DDX27 Antibody (C-term) AP11105b
    DDX27 Antibody (C-term) (Cat. #AP11105b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q96GQ7
Other Accession A1A4H6, NP_060365.7
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 89835 Da
Additional info
Gene ID 55661
Other Names Probable ATP-dependent RNA helicase DDX27, DEAD box protein 27, DDX27, RHLP
Target/Specificity This DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-796 amino acids from the C-terminal region of human DDX27.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDDX27 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX27
Synonyms cPERP-F {ECO:0000303|PubMed:20813266}, R
Function Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA (PubMed:25825154).
Cellular Location Nucleus, nucleolus. Chromosome. Note=Associates with 60S and 90S pre-ribosomal particles (PubMed:25825154)
Research Areas

BACKGROUND

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq].

REFERENCES

Deloukas, P., et al. Nature 414(6866):865-871(2001)

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