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CYP2B6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 3 - CYP2B6 Antibody (Center) AP11104C
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling CYP2B6 with AP11104C at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing endosomes staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
  • 1 - CYP2B6 Antibody (Center) AP11104C
    All lanes : Anti-CYP2B6 Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CYP2B6 Antibody (Center) AP11104C
    CYP2B6 Antibody (Center) (Cat. #AP11104c)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2B6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, IHC-P, E
Primary Accession P20813
Other Accession NP_000758.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56278 Da
Additional info
Gene ID 1555
Other Names Cytochrome P450 2B6, 11413-, 4-cineole 2-exo-monooxygenase, CYPIIB6, Cytochrome P450 IIB1, CYP2B6
Target/Specificity This CYP2B6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human CYP2B6.
Dilution IF~~1:25
WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP2B6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP2B6
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Tissue Location Expressed in liver, lung and heart right ventricle.
Research Areas
Characterization of feline cytochrome P450 2B6.
Author : Okamatsu G1, Komatsu T1, Ono Y1, Inoue H1, Uchide T1, Onaga T1, Endoh D1, Kitazawa T1, Hiraga T1, Uno Y2, Teraoka H1.
Xenobiotica. 2016 Mar 9:1-10. [Epub ahead of print]
26956662
The role of cytochromes p450 and aldo-keto reductases in prognosis of breast carcinoma patients.
Author : Hlaváč V1, Brynychová V, Václavíková R, Ehrlichová M, Vrána D, Pecha V, Trnková M, Kodet R, Mrhalová M, Kubáčková K, Gatěk J, Vážan P, Souček P.
Medicine (Baltimore). 2014 Dec;93(28):e255. doi: 10.1097/MD.0000000000000255.
25526449

BACKGROUND

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

REFERENCES

Figueroa, S.C., et al. Ther Drug Monit 32(5):579-585(2010)
Chou, M., et al. Antimicrob. Agents Chemother. 54(10):4432-4439(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Bunten, H., et al. Clin. Pharmacol. Ther. 88(3):383-389(2010)
Gounden, V., et al. AIDS Res Ther 7, 32 (2010) :

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