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CCNB1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CCNB1 Antibody (C-term) AP11102b
    Anti-CCNB1 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CCNB1 Antibody (C-term) AP11102b
    CCNB1 Antibody (C-term) (Cat. #AP11102b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CCNB1 antibody detected the CCNB1 protein (arrow).
  • 14 - CCNB1 Antibody (C-term) AP11102b
    CCNB1 Antibody (C-term) (Cat. #AP11102b)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P14635
Other Accession NP_114172.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48337 Da
Additional info
Gene ID 891
Other Names G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Target/Specificity This CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-411 amino acids from the C-terminal region of human CCNB1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCNB1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNB1
Synonyms CCNB
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Research Areas

BACKGROUND

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].

REFERENCES

Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)
van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)
Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Nantajit, D., et al. PLoS ONE 5 (8), E12341 (2010) :

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