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HDAC9 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HDAC9 Antibody (N-term) AP1109A
    Both anti-HDAC9 N-term (AP1109a) and C-term (AP1109b) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.
  • 5 - HDAC9 Antibody (N-term) AP1109A
    This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).
  • 1 - HDAC9 Antibody (N-term) AP1109A
    Figure 1: Immunoblots for MITR (AP1109a HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
  • 3 - HDAC9 Antibody (N-term) AP1109A
    Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a FLAG antibody (bottom panel) detecting a FLAG epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
  • 14 - HDAC9 Antibody (N-term) AP1109A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IP, IF, IHC-P, E
Primary Accession Q9UKV0
Other Accession Q99N13, Q5ZKH6
Reactivity Human
Predicted Chicken, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 111297 Da
Additional info
Gene ID 9734
Other Names Histone deacetylase 9, HD9, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR
Target/Specificity This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human HDAC9.
Dilution WB~~1:1000
IP~~1:100
IF~~1:1,000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHDAC9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HDAC9
Synonyms HDAC7, HDAC7B, HDRP, KIAA0744, MITR
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
Cellular Location Nucleus.
Tissue Location Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level)
Research Areas
Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement.
Author : Salian-Mehta S1, Xu M1, McKinsey TA2, Tobet S3, Wierman ME4.
J Biol Chem. 2015 May 29;290(22):14045-56. doi: 10.1074/jbc.M115.640482. Epub 2015 Apr 14.
25873389
Nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons.
Author : Sugo N, Oshiro H, Takemura M, Kobayashi T, Kohno Y, Uesaka N, Song WJ, Yamamoto N.
Eur J Neurosci. 2010 May;31(9):1521-32. doi: 10.1111/j.1460-9568.2010.07218.x.
20525066
Mirk/dyrk1B decreases the nuclear accumulation of class II histone deacetylases during skeletal muscle differentiation.
Author : Deng X, Ewton DZ, Mercer SE, Friedman E.
J Biol Chem. 2005 Feb 11;280(6):4894-905. Epub 2004 Nov 16.
15546868

BACKGROUND

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.

REFERENCES

Petrie, K., et al., J. Biol. Chem. 278(18):16059-16072 (2003). David, D., et al., Genomics 81(5):489-503 (2003). Mahlknecht, U., et al., Biochem. Biophys. Res. Commun. 293(1):182-191 (2002). Zhou, X., et al., Proc. Natl. Acad. Sci. U.S.A. 98(19):10572-10577 (2001). Zhang, C.L., et al., J. Biol. Chem. 276(1):35-39 (2001).

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