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GNAT2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - GNAT2 Antibody (Center) AP11077C
    Anti-GNAT2 Antibody (Center) at 1:1000 dilution + Y79 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GNAT2 Antibody (Center) AP11077C
    GNAT2 Antibody (Center) (Cat. #AP11077c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the GNAT2 antibody detected the GNAT2 protein (arrow).
  • 14 - GNAT2 Antibody (Center) AP11077C
    GNAT2 antibody (Center) (Cat. #AP11077c) immunohistochemistry analysis in formalin fixed and paraffin embedded human Kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GNAT2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P19087
Other Accession NP_005263.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40176 Da
Additional info
Gene ID 2780
Other Names Guanine nucleotide-binding protein G(t) subunit alpha-2, Transducin alpha-2 chain, GNAT2, GNATC
Target/Specificity This GNAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-169 amino acids from the Central region of human GNAT2.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNAT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNAT2
Synonyms GNATC
Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
Tissue Location Retinal rod outer segment.
Research Areas
Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells.
Author : Liszt KI1,2,Ley JP3,Lieder B1,2,Behrens M4,Stöger V2,Reiner A5,Hochkogler CM2,Köck E1,Marchiori A4,Hans J3,Widder S3,Krammer G3,Sanger GJ6,Somoza MM7,Meyerhof W4,Somoza V8,2.
Proc Natl Acad Sci U S A. 2017 Jul 10. pii: 201703728. doi: 10.1073/pnas.1703728114. [Epub ahead of print]
28696284

BACKGROUND

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in cones.

REFERENCES

Shi, J., et al. Mol. Psychiatry (2010) In press :
Thiadens, A.A., et al. Ophthalmology 116(10):1984-1989(2009)
Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)
Oldham, W.M., et al. Nat. Struct. Mol. Biol. 13(9):772-777(2006)
Rosenberg, T., et al. Invest. Ophthalmol. Vis. Sci. 45(12):4256-4262(2004)

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