- 文献引用 : 4
|Application ||IHC-P, WB, E|
|Calculated MW||131419 Da|
|Other Names||Histone deacetylase 6, HD6, HDAC6, KIAA0901|
|Target/Specificity||This HDAC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1182-1215 amino acids from the C-terminal region of human HDAC6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HDAC6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.|
|Cellular Location||Nucleus. Cytoplasm. Perikaryon. Cell projection, dendrite. Cell projection, axon. Note=It is mainly cytoplasmic, where it is associated with microtubules|
Author : Izumi H1,Li Y2,Shibaki M3,Mori D3,Yasunami M3,Sato S4,Matsunaga H4,Mae T4,Kodama K4,Kamijo T5,Kaneko Y5,Nakagawara A4,6.
Sci Rep. 2019 Feb 19;9(1):2236. doi: 10.1038/s41598-019-39229-8.
Author : Ran J1, Yang Y1, Li D1, Liu M1, Zhou J1.
Sci Rep. 2015 Aug 6;5:12917. doi: 10.1038/srep12917.
Author : Yu H1,Diao H1,Wang C2,Lin Y3,Yu F4,Lu H5,Xu W3,Li Z5,Shi H1,Zhao S6,Zhou Y7,Zhang Y8.
Mol Cell Proteomics. 2015 Apr;14(4):1009-23. doi: 10.1074/mcp.M114.041384. Epub 2015 Feb 13.
Author : Wang G1, Chen Q, Zhang X, Zhang B, Zhuo X, Liu J, Jiang Q, Zhang C.
J Cell Sci. 2013 Mar 15;126(Pt 6):1355-65. doi: 10.1242/jcs.114918. Epub 2013 Jan 23.
Provided below are standard protocols that you may find useful for product applications.
HDAC6 (histone deacetylase 6) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC6 plays a central role in microtubule-dependent cell motility via deacetylation of tubulin, and has been shown to interact with HDAC11, SIRT2, and F-actin. HDAC6 is ubiquitinated, but its polyubiquitination however does not lead to degradation. HDAC is also a potential target of sumoylation.
Hook, S.S., et al., Proc. Natl. Acad. Sci. U.S.A. 99(21):13425-13430 (2002).
Grozinger, C.M., et al., Proc. Natl. Acad. Sci. U.S.A. 96(9):4868-4873 (1999).
Wolffe, A.P., Nature 387(6628):16-17 (1997).
Pazin, M.J., et al., Cell 89(3):325-328 (1997).
Mahlknecht, U., et al., Cytogenet. Cell Genet. 93 (1-2), 135-136 (2001).