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>   首页   >   产品   >   一抗   >   精选抗体   >   细胞自噬抗体   >   HDAC6 Antibody (C-term)   

HDAC6 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HDAC6 Antibody (C-term) AP1106a
    All lanes : Anti-HDAC6 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 131 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - HDAC6 Antibody (C-term) AP1106a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HDAC6 Antibody (C-term)(Cat.#AP1106a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9UBN7
Other Accession NP_006035
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 131419 Da
Additional info
Gene ID 10013
Other Names Histone deacetylase 6, HD6, HDAC6, KIAA0901
Target/Specificity This HDAC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1182-1215 amino acids from the C-terminal region of human HDAC6.
Dilution WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHDAC6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HDAC6
Synonyms KIAA0901
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
Cellular Location Nucleus. Cytoplasm. Perikaryon. Cell projection, dendrite. Cell projection, axon. Note=It is mainly cytoplasmic, where it is associated with microtubules
Research Areas
Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region.
Author : Izumi H1,Li Y2,Shibaki M3,Mori D3,Yasunami M3,Sato S4,Matsunaga H4,Mae T4,Kodama K4,Kamijo T5,Kaneko Y5,Nakagawara A4,6.
Sci Rep. 2019 Feb 19;9(1):2236. doi: 10.1038/s41598-019-39229-8.
30783186
Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly.
Author : Ran J1, Yang Y1, Li D1, Liu M1, Zhou J1.
Sci Rep. 2015 Aug 6;5:12917. doi: 10.1038/srep12917.
26246421
Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).
Author : Yu H1,Diao H1,Wang C2,Lin Y3,Yu F4,Lu H5,Xu W3,Li Z5,Shi H1,Zhao S6,Zhou Y7,Zhang Y8.
Mol Cell Proteomics. 2015 Apr;14(4):1009-23. doi: 10.1074/mcp.M114.041384. Epub 2015 Feb 13.
25680958
PCM1 recruits Plk1 to the pericentriolar matrix to promote primary cilia disassembly before mitotic entry.
Author : Wang G1, Chen Q, Zhang X, Zhang B, Zhuo X, Liu J, Jiang Q, Zhang C.
J Cell Sci. 2013 Mar 15;126(Pt 6):1355-65. doi: 10.1242/jcs.114918. Epub 2013 Jan 23.
23345402

BACKGROUND

HDAC6 (histone deacetylase 6) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC6 plays a central role in microtubule-dependent cell motility via deacetylation of tubulin, and has been shown to interact with HDAC11, SIRT2, and F-actin. HDAC6 is ubiquitinated, but its polyubiquitination however does not lead to degradation. HDAC is also a potential target of sumoylation.

REFERENCES

Hook, S.S., et al., Proc. Natl. Acad. Sci. U.S.A. 99(21):13425-13430 (2002).
Grozinger, C.M., et al., Proc. Natl. Acad. Sci. U.S.A. 96(9):4868-4873 (1999).
Wolffe, A.P., Nature 387(6628):16-17 (1997).
Pazin, M.J., et al., Cell 89(3):325-328 (1997).
Mahlknecht, U., et al., Cytogenet. Cell Genet. 93 (1-2), 135-136 (2001).

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