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ENASE Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ENASE Antibody  (Center) AP11055c
    All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: human brain tissue lysate Lane 3: mouse thymus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ENASE Antibody  (Center) AP11055c
    ENASE Antibody (Center) (Cat. #AP11055c) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ENASE Antibody  (Center) AP11055c
    ENASE Antibody (Center) (Cat. #AP11055c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q8NFI3
Other Accession Q8BX80, NP_001036038.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 83987 Da
Additional info
Gene ID 64772
Other Names Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE
Target/Specificity This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENASE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENGASE
Function Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
Cellular Location Cytoplasm, cytosol.
Tissue Location Widely expressed. Expressed at higher level in thymus and spleen.
Research Areas

BACKGROUND

Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol.

REFERENCES

Park, K., et al. Diabetes 59(7):1845-1850(2010)
Dorfmueller, H.C., et al. FEBS Lett. 584(4):694-700(2010)
Chantret, I., et al. PLoS ONE 5 (7), E11734 (2010) :
Butkinaree, C., et al. J. Biol. Chem. 283(35):23557-23566(2008)
Ortutay, Z., et al. Arthritis Rheum. 48(8):2163-2172(2003)

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