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OTUD3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - OTUD3 Antibody (Center) AP11031C
    Immunohistochemical analysis of paraffin-embedded human testis tissue using AP11031C performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - OTUD3 Antibody (Center) AP11031C
    Immunohistochemical analysis of paraffin-embedded human brain tissue using AP11031C performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - OTUD3 Antibody (Center) AP11031C
    All lanes : Anti-OTUD3 Antibody (Center) at 1:2000 dilution Lane 1: LNCaP whole cell lysate Lane 2: RD whole cell lysate Lane 3: 293 whole cell lysate Lane 4: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - OTUD3 Antibody (Center) AP11031C
    Anti-OTUD3 Antibody (Center) at 1:2000 dilution + Caco2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q5T2D3
Other Accession NP_056022.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 23252
Other Names OTU domain-containing protein 3, OTUD3, KIAA0459
Target/Specificity This OTUD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human OTUD3.
Dilution IHC-P-Leica~~1:500
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOTUD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OTUD3
Synonyms KIAA0459
Function Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains.
Research Areas

REFERENCES

McGovern, D.P., et al. Nat. Genet. 42(4):332-337(2010)
Franke, A., et al. Nat. Genet. 42(4):292-294(2010)
Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009)
Silverberg, M.S., et al. Nat. Genet. 41(2):216-220(2009)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)

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