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NUDT15 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NUDT15 Antibody (C-term) AP11030b
    Anti-NUDT15 Antibody (C-term) at 1:500 dilution + Mouse small intestine tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NUDT15 Antibody (C-term) AP11030b
    Anti-NUDT15 Antibody (C-term) at 1:1000 dilution + SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NUDT15 Antibody (C-term) AP11030b
    Anti-NUDT15 Antibody (C-term) at 1:1000 dilution + HuTu80 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - NUDT15 Antibody (C-term) AP11030b
    NUDT15 antibody (C-term) (Cat. #AP11030b) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUDT15 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - NUDT15 Antibody (C-term) AP11030b
    NUDT15 Antibody (C-term) (Cat. #AP11030b) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q9NV35
Other Accession NP_060753.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18609 Da
Additional info
Gene ID 55270
Other Names Probable 8-oxo-dGTP diphosphatase NUDT15, 8-oxo-dGTPase NUDT15, 8-dihydro-8-oxoguanine-triphosphatase NUDT15, MutT homolog 2, MTH2, Nucleoside diphosphate-linked moiety X motif 15, Nudix motif 15, NUDT15, MTH2
Target/Specificity This NUDT15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the C-terminal region of human NUDT15.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUDT15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUDT15
Synonyms MTH2
Function Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear (By similarity). May have a role in DNA synthesis and cell cycle progression through the interaction with PCNA.
Research Areas

BACKGROUND

Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear (By similarity). May have a role in DNA synthesis and cell cycle progression throught the interaction with PCNA.

REFERENCES

Hori, M., et al. Free Radic. Biol. Med. 48(9):1197-1201(2010) Yu, Y., et al. J. Biol. Chem. 284(29):19310-19320(2009) Dunham, A., et al. Nature 428(6982):522-528(2004) Cai, J.P., et al. Biochem. Biophys. Res. Commun. 305(4):1073-1077(2003)

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