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HEMK1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HEMK1 Antibody (C-term) AP11010b
    HEMK1 Antibody (C-term) (Cat. #AP11010b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HEMK1 antibody detected the HEMK1 protein (arrow).
  • 14 - HEMK1 Antibody (C-term) AP11010b
    HEMK1 antibody (C-term) (Cat. #AP11010b) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph node followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HEMK1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - HEMK1 Antibody (C-term) AP11010b
    HEMK1 Antibody (C-term) (Cat. #AP11010b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q9Y5R4
Other Accession NP_057257.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38231 Da
Additional info
Gene ID 51409
Other Names HemK methyltransferase family member 1, 211-, MHsaHemKP, HEMK1, HEMK
Target/Specificity This HEMK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-338 amino acids from the C-terminal region of human HEMK1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHEMK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HEMK1
Synonyms HEMK
Function N5-glutamine methyltransferase responsible for the methylation of the GGQ triplet of the mitochondrial translation release factor MTRF1L.
Cellular Location Mitochondrion.
Research Areas

BACKGROUND

Probable protein methyltransferase. May methylate a Gln residue in target proteins (By similarity).

REFERENCES

Ishizawa, T., et al. Biochem. Biophys. Res. Commun. 373(1):99-103(2008)
Bujnicki, J.M., et al. IUBMB Life 48(3):247-249(1999)

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