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ASAP3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ASAP3 Antibody  (N-term) AP11003a
    ASAP3 Antibody (N-term) (Cat. #AP11003a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).
  • 1 - ASAP3 Antibody  (N-term) AP11003a
    ASAP3 Antibody (N-term) (Cat. #AP11003a) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).
  • 14 - ASAP3 Antibody  (N-term) AP11003a
    ASAP3 antibody (N-term) (Cat. #AP11003a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASAP3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q8TDY4
Other Accession NP_001137250.1, NP_060177.2
Reactivity Human, Hamster
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, ASAP3, DDEFL1, UPLC1
Target/Specificity This ASAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human ASAP3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASAP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASAP3
Synonyms DDEFL1, UPLC1
Function Promotes cell proliferation.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes EMBL; AB056749; BAB85677.1; -; mRNA EMBL; AK000206; BAA91008.1; -; mRNA EMBL; AK092291; BAG52522.1; -; mRNA EMBL; AK295100; BAG58136.1; -; mRNA EMBL; AL021154; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AL357134; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471134; EAW95050.1; -; Genomic_DNA EMBL; BC052305; AAH52305.1; -; mRNA EMBL; BC060786; AAH60786.1; -; mRNA CCDS; CCDS235.1; -. [Q8TDY4-1] CCDS; CCDS44087.1; -. [Q8TDY4-3] RefSeq; NP_001137250.1; NM_001143778.1. [Q8TDY4-3] RefSeq; NP_060177.2; NM_017707.3. [Q8TDY4-1] UniGene; Hs.437379; - PDB; 2B0O; X-ray; 2.06 A; E/F/G/H=416-697 PDB; 3LVQ; X-ray; 3.38 A; E=416-697 PDB; 3LVR; X-ray; 3.38 A; E=416-697 PDBsum; 2B0O; - PDBsum; 3LVQ; - PDBsum; 3LVR; - ProteinModelPortal; Q8TDY4; - SMR; Q8TDY4; - BioGrid; 120756; 17 IntAct; Q8TDY4; 8 STRING; 9606.ENSP00000338769; - iPTMnet; Q8TDY4; - PhosphoSitePlus; Q8TDY4; - BioMuta; ASAP3; - DMDM; 74751433; - EPD; Q8TDY4; - jPOST; Q8TDY4; - MaxQB; Q8TDY4; - PaxDb; Q8TDY4; - PeptideAtlas; Q8TDY4; - PRIDE; Q8TDY4; - ProteomicsDB; 74371; - ProteomicsDB; 74372; -. [Q8TDY4-2] ProteomicsDB; 74373; -. [Q8TDY4-3] Ensembl; ENST00000336689; ENSP00000338769; ENSG00000088280. [Q8TDY4-1] Ensembl; ENST00000437606; ENSP00000408826; ENSG00000088280. [Q8TDY4-3] Ensembl; ENST00000495646; ENSP00000436150; ENSG00000088280. [Q8TDY4-2] Ensembl; ENST00000634513; ENSP00000488934; ENSG00000282854. [Q8TDY4-2] Ensembl; ENST00000634561; ENSP00000488946; ENSG00000282854. [Q8TDY4-1] Ensembl; ENST00000635198; ENSP00000488953; ENSG00000282854. [Q8TDY4-3] GeneID; 55616; - KEGG; hsa:55616; - UCSC; uc001bgy.1; human. [Q8TDY4-1] CTD; 55616; - DisGeNET; 55616; - EuPathDB; HostDB:ENSG00000088280.18; - GeneCards; ASAP3; - H-InvDB; HIX0000247; - HGNC; HGNC:14987; ASAP3 HPA; HPA020537; - HPA; HPA020546; - MIM; 616594; gene neXtProt; NX_Q8TDY4; - OpenTargets; ENSG00000088280; - PharmGKB; PA164716179; - eggNOG; KOG0521; Eukaryota eggNOG; COG5347; LUCA GeneTree; ENSGT00940000161085; - HOGENOM; HOG000230570; - HOVERGEN; HBG051327; - InParanoid; Q8TDY4; - KO; K12488; - OMA; STRRDYI; - OrthoDB; 751525at2759; - PhylomeDB; Q8TDY4; - TreeFam; TF325156; - ChiTaRS; ASAP3; human EvolutionaryTrace; Q8TDY4; - GenomeRNAi; 55616; - PRO; PR:Q8TDY4; - Proteomes; UP000005640; Chromosome 1 Bgee; ENSG00000088280; Expressed in 206 organ(s), highest expression level in right ovary ExpressionAtlas; Q8TDY4; baseline and differential Genevisible; Q8TDY4; HS GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0005925; C:focal adhesion; IDA:UniProtKB GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0001726; C:ruffle; IDA:UniProtKB GO; GO:0005096; F:GTPase activator activity; IDA:UniProtKB GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0016477; P:cell migration; IMP:UniProtKB GO; GO:0043547; P:positive regulation of GTPase activity; IDA:UniProtKB GO; GO:0051492; P:regulation of stress fiber assembly; IMP:UniProtKB CDD; cd00204; ANK; 1 CDD; cd13251; PH_ASAP; 1 Gene3D; 1.20.1270.60; -; 1 Gene3D; 2.30.29.30; -; 1 Gene3D; 3.30.40.160; -; 1 InterPro; IPR027267; AH/BAR_dom_sf InterPro; IPR002110; Ankyrin_rpt InterPro; IPR020683; Ankyrin_rpt-contain_dom InterPro; IPR036770; Ankyrin_rpt-contain_sf InterPro; IPR037278; ARFGAP/RecO InterPro; IPR001164; ArfGAP_dom InterPro; IPR038508; ArfGAP_dom_sf InterPro; IPR028775; ASAP3 InterPro; IPR011993; PH-like_dom_sf InterPro; IPR037844; PH_ASAP InterPro; IPR001849; PH_domain PANTHER; PTHR23180:SF271; PTHR23180:SF271; 1 Pfam; PF12796; Ank_2; 1 Pfam; PF01412; ArfGap; 1 Pfam; PF00169; PH; 1 PRINTS; PR00405; REVINTRACTNG SMART; SM00248; ANK; 2 SMART; SM00105; ArfGap; 1 SMART; SM00233; PH; 1 SUPFAM; SSF103657; SSF103657; 1 SUPFAM; SSF48403; SSF48403; 1 SUPFAM; SSF57863; SSF57863; 1 PROSITE; PS50297; ANK_REP_REGION; 1 PROSITE; PS50088; ANK_REPEAT; 1 PROSITE; PS50115; ARFGAP; 1 PROSITE; PS50003; PH_DOMAIN; 1 1: Evidence at protein level; 3D-structure; Alternative splicing; ANK repeat; Coiled coil; Complete proteome; Cytoplasm; Metal-binding; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger CHAIN 1 903 Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 /FTId=PRO_0000232887 DOMAIN 302 394 PH. {ECO:0000255|PROSITE- ProRule:PRU00145} DOMAIN 426 551 Arf-GAP. {ECO:0000255|PROSITE- ProRule:PRU00288} REPEAT 585 617 ANK 1 REPEAT 621 650 ANK 2 ZN_FING 441 464 C4-type. {ECO:0000255|PROSITE- ProRule:PRU00288} COILED 141 172 COILED 248 275 COMPBIAS 798 801 Poly-Ser VAR_SEQ 1 496 Missing (in isoform 2) /FTId=VSP_018013 VAR_SEQ 142 158 DSKKQLEKAWKDYEAKM -> ASLSLGSR (in isoform 3). /FTId=VSP_039827 VARIANT 377 377 E -> A (in dbSNP:rs16828486) /FTId=VAR_048295 VARIANT 617 617 A -> T (in a colorectal cancer sample; somatic mutation) /FTId=VAR_035612 CONFLICT 319 319 V -> A (in Ref. 5; AAH60786) CONFLICT 521 521 E -> G (in Ref. 2; BAA91008) CONFLICT 596 596 V -> D (in Ref. 2; BAA91008) CONFLICT 802 802 L -> V (in Ref. 2; BAA91008) HELIX 424 433 {ECO:0000244|PDB:2B0O} TURN 436 439 {ECO:0000244|PDB:2B0O} TURN 442 444 {ECO:0000244|PDB:2B0O} STRAND 451 453 {ECO:0000244|PDB:2B0O} TURN 454 456 {ECO:0000244|PDB:2B0O} HELIX 462 471 {ECO:0000244|PDB:2B0O} TURN 473 475 {ECO:0000244|PDB:2B0O} STRAND 478 480 {ECO:0000244|PDB:2B0O} TURN 481 483 {ECO:0000244|PDB:2B0O} HELIX 488 496 {ECO:0000244|PDB:2B0O} HELIX 499 506 {ECO:0000244|PDB:2B0O} TURN 507 509 {ECO:0000244|PDB:2B0O} STRAND 512 514 {ECO:0000244|PDB:2B0O} HELIX 524 535 {ECO:0000244|PDB:2B0O} HELIX 548 556 {ECO:0000244|PDB:2B0O} HELIX 560 568 {ECO:0000244|PDB:2B0O} STRAND 573 575 {ECO:0000244|PDB:3LVQ} STRAND 580 582 {ECO:0000244|PDB:2B0O} HELIX 589 595 {ECO:0000244|PDB:2B0O} TURN 599 601 {ECO:0000244|PDB:2B0O} HELIX 602 611 {ECO:0000244|PDB:2B0O} STRAND 620 622 {ECO:0000244|PDB:3LVQ} HELIX 625 631 {ECO:0000244|PDB:2B0O} HELIX 635 643 {ECO:0000244|PDB:2B0O} HELIX 658 665 {ECO:0000244|PDB:2B0O} HELIX 668 680 {ECO:0000244|PDB:2B0O} HELIX 690 696 {ECO:0000244|PDB:2B0O} SEQUENCE 903 AA; 99155 MW; D5E89518AFC86656 CRC64; MPEQFSVAEF LAVTAEDLSS PAGAAAFAAK MPRYRGAALA REEILEGDQA ILQRIKKAVR AIHSSGLGHV ENEEQYREAV ESLGNSHLSQ NSHELSTGFL NLAVFTREVA ALFKNLIQNL NNIVSFPLDS LMKGQLRDGR QDSKKQLEKA WKDYEAKMAK LEKERDRARV TGGIPGEVAQ DMQRERRIFQ LHMCEYLLKA GESQMKQGPD FLQSLIKFFH AQHNFFQDGW KAAQSLFPFI EKLAASVHAL HQAQEDELQK LTQLRDSLRG TLQLESREEH LSRKNSGCGY SIHQHQGNKQ FGTEKVGFLY KKSDGIRRVW QKRKCGVKYG CLTISHSTIN RPPVKLTLLT CQVRPNPEEK KCFDLVTHNR TYHFQAEDEH ECEAWVSVLQ NSKDEALSSA FLGEPSAGPG SWGSAGHDGE PHDLTKLLIA EVKSRPGNSQ CCDCGAADPT WLSTNLGVLT CIQCSGVHRE LGVRFSRMQS LTLDLLGPSE LLLALNMGNT SFNEVMEAQL PSHGGPKPSA ESDMGTRRDY IMAKYVEHRF ARRCTPEPQR LWTAICNRDL LSVLEAFANG QDFGQPLPGP DAQAPEELVL HLAVKVANQA SLPLVDFIIQ NGGHLDAKAA DGNTALHYAA LYNQPDCLKL LLKGRALVGT VNEAGETALD IARKKHHKEC EELLEQAQAG TFAFPLHVDY SWVISTEPGS DSEEDEEEKR CLLKLPAQAH WASGRLDISN KTYETVASLG AATPQGESED CPPPLPVKNS SRTLVQGCAR HASGDRSEVS SLSSEAPETP ESLGSPASSS SLMSPLEPGD PSQAPPNSEE GLREPPGTSR PSLTSGTTPS EMYLPVRFSS ESTRSYRRGA RSPEDGPSAR QPLPRRNVPV GITEGDGSRT GSLPASSVQL LQD
Research Areas

BACKGROUND

This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants.

REFERENCES

Ismail, S.A., et al. Cell 141(5):812-821(2010)
Ha, V.L., et al. J. Biol. Chem. 283(22):14915-14926(2008)
Fang, Z., et al. Mol. Cell Proteomics 5(8):1437-1449(2006)
Okabe, H., et al. Int. J. Oncol. 24(1):43-48(2004)

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