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ASAP3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ASAP3 Antibody  (N-term) AP11003a
    ASAP3 Antibody (N-term) (Cat. #AP11003a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).
  • 1 - ASAP3 Antibody  (N-term) AP11003a
    ASAP3 Antibody (N-term) (Cat. #AP11003a) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the ASAP3 antibody detected the ASAP3 protein (arrow).
  • 14 - ASAP3 Antibody  (N-term) AP11003a
    ASAP3 antibody (N-term) (Cat. #AP11003a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASAP3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q8TDY4
Other Accession NP_001137250.1, NP_060177.2
Reactivity Human, Hamster
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 99155 Da
Additional info
Gene ID 55616
Other Names Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, ASAP3, DDEFL1, UPLC1
Target/Specificity This ASAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human ASAP3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASAP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASAP3
Synonyms DDEFL1, UPLC1
Function Promotes cell proliferation.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes
Research Areas

BACKGROUND

This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants.

REFERENCES

Ismail, S.A., et al. Cell 141(5):812-821(2010)
Ha, V.L., et al. J. Biol. Chem. 283(22):14915-14926(2008)
Fang, Z., et al. Mol. Cell Proteomics 5(8):1437-1449(2006)
Okabe, H., et al. Int. J. Oncol. 24(1):43-48(2004)

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