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NDUF3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NDUF3 Antibody (N-term) AP10989a
    Anti-NDUF3 Antibody (N-term) at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - NDUF3 Antibody (N-term) AP10989a
    NDUF3 antibody (N-term) (Cat. #AP10989a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUF3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9BU61
Other Accession NP_951032.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20350 Da
Additional info
Gene ID 25915
Other Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3, NDUFAF3, C3orf60
Target/Specificity This NDUF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human NDUF3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUF3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFAF3
Synonyms C3orf60
Function Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
Cellular Location Nucleus. Mitochondrion inner membrane
Research Areas

BACKGROUND

This gene encodes a mitochondrial complex I assembly protein that interacts with complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency, a fatal neonatal disorder of the oxidative phosphorylation system. Alternatively spliced transcript variants encoding different isoforms have been identified.

REFERENCES

Saada, A., et al. Am. J. Hum. Genet. 84(6):718-727(2009)
Hammami-Hamza, S., et al. Int. J. Dev. Biol. 47(1):71-76(2003)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)

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