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CD2BP2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CD2BP2 Antibody (N-term) AP10949a
    CD2BP2 Antibody (N-term) (Cat. #AP10949a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CD2BP2 antibody detected the CD2BP2 protein (arrow).
  • 14 - CD2BP2 Antibody (N-term) AP10949a
    CD2BP2 antibody(N-term) (Cat. #AP10949a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD2BP2 antibody(N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - CD2BP2 Antibody (N-term) AP10949a
    CD2BP2 Antibody (N-term) (Cat. #AP10949a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession O95400
Other Accession Q9CWK3, NP_006101.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names CD2 antigen cytoplasmic tail-binding protein 2, CD2 cytoplasmic domain-binding protein 2, CD2 tail-binding protein 2, U5 snRNP 52K protein, U5-52K, CD2BP2, KIAA1178
Target/Specificity This CD2BP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human CD2BP2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD2BP2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD2BP2
Synonyms KIAA1178
Function Involved in pre-mRNA splicing as component of the U5 snRNP complex that is involved in spliceosome assembly.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly nuclear EMBL; AF104222; AAC84141.1; -; mRNA EMBL; AK315708; BAG38069.1; -; mRNA EMBL; CH471192; EAW52260.1; -; Genomic_DNA EMBL; BC000495; AAH00495.1; -; mRNA EMBL; BC001947; AAH01947.1; -; mRNA EMBL; AB033004; BAA86492.1; -; mRNA CCDS; CCDS10675.1; - RefSeq; NP_001230575.1; NM_001243646.1 RefSeq; NP_006101.1; NM_006110.2 UniGene; Hs.202677; - PDB; 1GYF; NMR; -; A=280-341 PDB; 1L2Z; NMR; -; A=280-341 PDB; 1SYX; X-ray; 2.34 A; B/D/F=262-341 PDB; 4BWS; X-ray; 2.50 A; C/F=280-341 PDBsum; 1GYF; - PDBsum; 1L2Z; - PDBsum; 1SYX; - PDBsum; 4BWS; - ProteinModelPortal; O95400; - SMR; O95400; - BioGrid; 115690; 146 CORUM; O95400; - ELM; O95400; - IntAct; O95400; 29 MINT; O95400; - STRING; 9606.ENSP00000304903; - iPTMnet; O95400; - PhosphoSitePlus; O95400; - BioMuta; CD2BP2; - EPD; O95400; - jPOST; O95400; - MaxQB; O95400; - PaxDb; O95400; - PeptideAtlas; O95400; - PRIDE; O95400; - ProteomicsDB; 50853; - DNASU; 10421; - Ensembl; ENST00000305596; ENSP00000304903; ENSG00000169217 Ensembl; ENST00000569466; ENSP00000456935; ENSG00000169217 GeneID; 10421; - KEGG; hsa:10421; - UCSC; uc002dxr.4; human CTD; 10421; - DisGeNET; 10421; - EuPathDB; HostDB:ENSG00000169217.8; - GeneCards; CD2BP2; - HGNC; HGNC:1656; CD2BP2 HPA; HPA041508; - HPA; HPA061309; - MIM; 604470; gene neXtProt; NX_O95400; - OpenTargets; ENSG00000169217; - PharmGKB; PA26209; - eggNOG; KOG2950; Eukaryota eggNOG; ENOG4111KF5; LUCA GeneTree; ENSGT00390000012483; - HOGENOM; HOG000007061; - HOVERGEN; HBG056920; - InParanoid; O95400; - KO; K13099; - OMA; DGHYHWN; - OrthoDB; 773475at2759; - PhylomeDB; O95400; - TreeFam; TF313042; - Reactome; R-HSA-72163; mRNA Splicing - Major Pathway ChiTaRS; CD2BP2; human EvolutionaryTrace; O95400; - GeneWiki; CD2BP2; - GenomeRNAi; 10421; - PRO; PR:O95400; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000169217; Expressed in 230 organ(s), highest expression level in anatomical system Genevisible; O95400; HS GO; GO:0005737; C:cytoplasm; TAS:ProtInc GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0001650; C:fibrillar center; IDA:HPA GO; GO:0016607; C:nuclear speck; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:BHF-UCL GO; GO:0005634; C:nucleus; IDA:BHF-UCL GO; GO:0005682; C:U5 snRNP; IDA:BHF-UCL GO; GO:0043021; F:ribonucleoprotein complex binding; IDA:BHF-UCL GO; GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome GO; GO:0010923; P:negative regulation of phosphatase activity; IDA:UniProtKB GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; NAS:BHF-UCL CDD; cd00072; GYF; 1 Gene3D; 3.30.1490.40; -; 1 InterPro; IPR039905; CD2BP2/Lin1 InterPro; IPR003169; GYF InterPro; IPR035445; GYF-like_dom_sf PANTHER; PTHR13138; PTHR13138; 1 Pfam; PF02213; GYF; 1 SMART; SM00444; GYF; 1 SUPFAM; SSF55277; SSF55277; 1 PROSITE; PS50829; GYF; 1 1: Evidence at protein level; 3D-structure; Acetylation; Complete proteome; Cytoplasm; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation CHAIN 1 341 CD2 antigen cytoplasmic tail-binding protein 2 /FTId=PRO_0000089437 DOMAIN 280 338 GYF. {ECO:0000255|PROSITE- ProRule:PRU00101} MOD_RES 44 44 N6-acetyllysine {ECO:0000250|UniProtKB:Q9CWK3} MOD_RES 46 46 Phosphoserine MOD_RES 49 49 Phosphoserine MOD_RES 118 118 Phosphoserine MOD_RES 194 194 Phosphoserine MOD_RES 195 195 Phosphoserine CROSSLNK 26 26 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) VARIANT 231 231 G -> D (in dbSNP:rs13330462) /FTId=VAR_050772 VARIANT 262 262 T -> I (in dbSNP:rs34391305) /FTId=VAR_050773 STRAND 282 293 {ECO:0000244|PDB:1SYX} STRAND 296 300 {ECO:0000244|PDB:1SYX} HELIX 301 309 {ECO:0000244|PDB:1SYX} STRAND 318 324 {ECO:0000244|PDB:1SYX} HELIX 332 334 {ECO:0000244|PDB:1SYX} HELIX 337 340 {ECO:0000244|PDB:1SYX} SEQUENCE 341 AA; 37646 MW; 8E9A7EE0C40474D5 CRC64; MPKRKVTFQG VGDEEDEDEI IVPKKKLVDP VAGSGGPGSR FKGKHSLDSD EEEDDDDGGS SKYDILASED VEGQEAATLP SEGGVRITPF NLQEEMEEGH FDADGNYFLN RDAQIRDSWL DNIDWVKIRE RPPGQRQASD SEEEDSLGQT SMSAQALLEG LLELLLPRET VAGALRRLGA RGGGKGRKGP GQPSSPQRLD RLSGLADQMV ARGNLGVYQE TRERLAMRLK GLGCQTLGPH NPTPPPSLDM FAEELAEEEL ETPTPTQRGE AESRGDGLVD VMWEYKWENT GDAELYGPFT SAQMQTWVSE GYFPDGVYCR KLDPPGGQFY NSKRIDFDLY T
Research Areas

BACKGROUND

This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined.

REFERENCES

Heinze, M., et al. Int. Immunol. 19(11):1313-1318(2007)
Nielsen, T.K., et al. J. Mol. Biol. 369(4):902-908(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Al-Dhaheri, M.H., et al. Steroids 71 (11-12), 966-978 (2006) :

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