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CASP5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CASP5 Antibody (Center) AP10944b
    CASP5 Antibody (Center) (Cat. #AP10944b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CASP5 antibody detected the CASP5 protein (arrow).
  • 14 - CASP5 Antibody (Center) AP10944b
    CASP5 Antibody (Center) (Cat. #AP10944b) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - CASP5 Antibody (Center) AP10944b
    CASP5 Antibody (Center) (Cat. #AP10944b) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P51878
Other Accession NP_001129582.1, NP_001129584.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49736 Da
Additional info
Gene ID 838
Other Names Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TY, Caspase-5 subunit p20, Caspase-5 subunit p10, CASP5, ICH3
Target/Specificity This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the Central region of human CASP5.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCASP5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP5
Synonyms ICH3
Function Mediator of programmed cell death (apoptosis).
Tissue Location Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle
Research Areas

BACKGROUND

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.

REFERENCES

Ulybina, Y.M., et al. Exp. Gerontol. 45(9):726-729(2010)
Notaridou, M., et al. Int. J. Cancer (2010) In press :
Kim, M.S., et al. APMIS 118(4):308-312(2010)
Liang, X.S., et al. Br. J. Haematol. 146(4):418-423(2009)
Eckhart, L., et al. Biochem. Biophys. Res. Commun. 348(2):682-688(2006)

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