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RPL27A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RPL27A Antibody (C-term) AP10937b
    RPL27A Antibody (C-term) (Cat. #AP10937b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).
  • 1 - RPL27A Antibody (C-term) AP10937b
    RPL27A Antibody (C-term) (Cat. #AP10937b) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).
  • 14 - RPL27A Antibody (C-term) AP10937b
    RPL27A Antibody (C-term) (Cat. #AP10937b) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL27A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - RPL27A Antibody (C-term) AP10937b
    RPL27A Antibody (C-term) (Cat. #AP10937b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P46776
Other Accession P18445, P14115, Q4R723, Q56K03, NP_000981.1
Reactivity Human, Hamster
Predicted Bovine, Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16561 Da
Additional info
Gene ID 6157
Other Names 60S ribosomal protein L27a, RPL27A
Target/Specificity This RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the C-terminal region of human RPL27A.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL27A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL27A
Research Areas
RPL27A is a target of miR-595 and may contribute to the myelodysplastic phenotype through ribosomal dysgenesis.
Author : Alkhatabi HA1,2,McLornan DP1,3,Kulasekararaj AG1,3,Malik F1,Seidl T1,Darling D1,Gaken J1,Mufti GJ1,3.
Oncotarget. 2016 Jul 26;7(30):47875-47890. doi: 10.18632/oncotarget.10293.
27374104

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.

REFERENCES

Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Mazumder, B., et al. Cell 115(2):187-198(2003)
Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002)
Bortoluzzi, S., et al. Bioinformatics 17(12):1152-1157(2001)
Kusuda, J., et al. Cytogenet. Cell Genet. 85 (3-4), 248-251 (1999) :

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