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P5F1B Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - P5F1B Antibody (C-term) AP10822b
    P5F1B Antibody (C-term) (Cat. #AP10822b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the P5F1B antibody detected the P5F1B protein (arrow).
  • 4 - P5F1B Antibody (C-term) AP10822b
    P5F1B Antibody (C-term) (Cat. #AP10822b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q06416
Other Accession Q9TSV5, P20263, Q01860, O97552, NP_001153014.1
Reactivity Human
Predicted Bovine, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38588 Da
Additional info
Gene ID 5462
Other Names Putative POU domain, class 5, transcription factor 1B, Oct4-pg1, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like, POU5F1B
Target/Specificity This P5F1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human P5F1B.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsP5F1B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU5F1B
Function Shows weak transcriptional activator activity.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000255|PROSITE-ProRule:PRU00530, ECO:0000269|PubMed:18949397}
Tissue Location Detected at the mRNA level in several cancer tissues (breast, uterine cervix, lung, thyroid gland, esophagus, colon, urinary bladder, and glioma), but absent in normal tissues
Research Areas

BACKGROUND

This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development.

REFERENCES

Kastler, S., et al. Prostate 70(6):666-674(2010)
White, K.L., et al. Twin Res Hum Genet 13(1):43-56(2010)
Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
Gudmundsson, J., et al. Nat. Genet. 41(10):1122-1126(2009)
Panagopoulos, I., et al. Oncol. Rep. 20(5):1029-1033(2008)

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