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>   首页   >   产品   >   一抗   >   其他   >   KAT1 (HAT1) Antibody (C-term)   

KAT1 (HAT1) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - KAT1 (HAT1) Antibody (C-term) AP1079b
    Western blot analysis of hHAT1-E404 (Cat. #AP1079b) in 293, HepG2, Jurkat cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab.
  • 14 - KAT1 (HAT1) Antibody (C-term) AP1079b
    Formalin-fixed and paraffin-embedded H.colon tissue reacted with KAT1 (HAT1) Antibody (C-term) (Cat#AP1079b).
  • 14 - KAT1 (HAT1) Antibody (C-term) AP1079b
    Formalin-fixed and paraffin-embedded H.tonsil tissue reacted with KAT1 (HAT1) Antibody (C-term) (Cat#AP1079b).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O14929
Other Accession Q5M939, Q8BY71
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 8520
Other Names Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1
Target/Specificity This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-419 amino acids from the C-terminal region of human KAT1 (HAT1).
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKAT1 (HAT1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAT1
Synonyms KAT1
Function Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
Cellular Location Isoform A: Nucleus matrix.
Research Areas

BACKGROUND

Histone acetylation, particularly of histone H4, has been proposed to play an important role in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and B motifs, which are present in many N-acetyltransferases, including those that acetylate substrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic 46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 and bound to core histones. The HAT1 holoenzyme acetylated newly synthesized but not nucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1 and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role in telomeric silencing.

REFERENCES

Gronroos, E., et al., Mol. Cell 10(3):483-493 (2002).
Makowski, A.M., et al., J. Biol. Chem. 276(47):43499-43502 (2001).
Cheung, P., et al., Mol. Cell 5(6):905-915 (2000).
Verreault, A., et al., Curr. Biol. 8(2):96-108 (1998).

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