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GSDMC Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - GSDMC Antibody  (Center) AP10771C
    Overlay histogram showing U-2OS cells stained with AP10771c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10771c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 4 - GSDMC Antibody  (Center) AP10771C
    Overlay histogram showing U-2OS cells stained with AP10771c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10771c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - GSDMC Antibody  (Center) AP10771C
    All lanes : Anti-GSDMC Antibody (Center) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: MKN45 whole cell lysate Lane 3: SK-MEL-2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GSDMC Antibody  (Center) AP10771C
    GSDMC Antibody (Center) (Cat. #AP10771c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the GSDMC antibody detected the GSDMC protein (arrow).
  • 14 - GSDMC Antibody  (Center) AP10771C
    GSDMC antibody (Center) (Cat. #AP10771c) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSDMC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - GSDMC Antibody  (Center) AP10771C
    GSDMC Antibody (Center) (Cat. #AP10771c) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, IHC-P, E
Primary Accession Q9BYG8
Other Accession NP_113603.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 56169
Other Names Gasdermin-C, Melanoma-derived leucine zipper-containing extranuclear factor, GSDMC, MLZE
Target/Specificity This GSDMC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human GSDMC.
Dilution FC~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSDMC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSDMC
Synonyms MLZE
Cellular Location Cytoplasm.
Tissue Location Expressed primarily in trachea and spleen.
Research Areas

REFERENCES

Birnbaum, S., et al. Nat. Genet. 41(4):473-477(2009)
Saeki, N., et al. Genes Chromosomes Cancer 48(3):261-271(2009)
Tamura, M., et al. Genomics 89(5):618-629(2007)
Watabe, K., et al. Jpn. J. Cancer Res. 92(2):140-151(2001)

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