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PDHA2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PDHA2 Antibody (Center) AP10756c
    PDHA2 Antibody (Center) (Cat. #AP10756c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PDHA2 antibody detected the PDHA2 protein (arrow).
  • 14 - PDHA2 Antibody (Center) AP10756c
    PDHA2 antibody (Center) (Cat. #AP10756c) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDHA2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P29803
Other Accession NP_005381.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42933 Da
Additional info
Gene ID 5161
Other Names Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial, PDHE1-A type II, PDHA2, PDHAL
Target/Specificity This PDHA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the Central region of human PDHA2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDHA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDHA2
Synonyms PDHAL
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Tissue Location Testis. Expressed in postmeiotic spermatogenic cells.
Research Areas

BACKGROUND

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

REFERENCES

Pinheiro, A., et al. Mol. Genet. Metab. 99(4):425-430(2010)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Caruso, M., et al. J. Biol. Chem. 276(48):45088-45097(2001)
Jacobia, S.J., et al. Arch. Biochem. Biophys. 395(1):121-128(2001)

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