|Application ||IHC-P, WB, E|
|Calculated MW||34464 Da|
|Other Names||Rab effector Noc2, No C2 domains protein, Rabphilin-3A-like protein, RPH3AL, NOC2|
|Target/Specificity||This RPH3AL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-237 amino acids from the C-terminal region of human RPH3AL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RPH3AL Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells (By similarity). Acts as a potential RAB3B effector protein in epithelial cells.|
|Cellular Location||Cytoplasm. Cytoplasmic vesicle, secretory vesicle membrane. Note=Recruited to the vesicle membrane in a GTP- and RAB3B-dependent manner in epithelial cells|
|Tissue Location||Moderate to high levels of expression in thyroid, ovary, stomach, heart, pancreas, skeletal muscle, kidney and liver. Also detected in epithelial cells|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Rose, J. Phd, et al. Mol. Med. (2010) In press : Shanmugam, C., et al. Biotech Histochem 84(2):39-45(2009) Katkoori, V.R., et al. Front. Biosci. 13, 1050-1061 (2008) : Matsumoto, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(22):8313-8318(2004) Manabe, S., et al. Biochem. Biophys. Res. Commun. 316(1):218-225(2004)