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H2AFY2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - H2AFY2 Antibody (N-term) AP10726a
    H2AFY2 Antibody (N-term) (Cat. #AP10726a) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the H2AFY2 antibody detected the H2AFY2 protein (arrow).
  • 4 - H2AFY2 Antibody (N-term) AP10726a
    H2AFY2 Antibody (N-term) (Cat. #AP10726a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q9P0M6
Other Accession Q8CCK0, NP_061119.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Core histone macro-H2A2, Histone macroH2A2, mH2A2, H2AFY2, MACROH2A2
Target/Specificity This H2AFY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-39 amino acids from the N-terminal region of human H2AFY2.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsH2AFY2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2AFY2
Synonyms MACROH2A2
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
Cellular Location Nucleus Chromosome Note=Enriched in inactive X chromosome chromatin (PubMed:11331621, PubMed:11262398) and in senescence-associated heterochromatin (PubMed:15621527). EMBL; AF336304; AAK52471.1; -; mRNA EMBL; AF151534; AAF72101.1; -; mRNA EMBL; AK022776; BAB14239.1; -; mRNA EMBL; AL731540; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC016172; AAH16172.1; -; mRNA CCDS; CCDS7296.1; - RefSeq; NP_061119.1; NM_018649.2 UniGene; Hs.499953; - PDB; 2XD7; X-ray; 2.09 A; A/B/C/D=177-369 PDB; 6FY5; X-ray; 1.65 A; A/B=178-372 PDBsum; 2XD7; - PDBsum; 6FY5; - ProteinModelPortal; Q9P0M6; - SMR; Q9P0M6; - BioGrid; 120686; 120 DIP; DIP-48563N; - IntAct; Q9P0M6; 14 MINT; Q9P0M6; - STRING; 9606.ENSP00000362352; - iPTMnet; Q9P0M6; - PhosphoSitePlus; Q9P0M6; - BioMuta; H2AFY2; - DMDM; 12585260; - EPD; Q9P0M6; - jPOST; Q9P0M6; - MaxQB; Q9P0M6; - PaxDb; Q9P0M6; - PeptideAtlas; Q9P0M6; - PRIDE; Q9P0M6; - ProteomicsDB; 83580; - DNASU; 55506; - Ensembl; ENST00000373255; ENSP00000362352; ENSG00000099284 GeneID; 55506; - KEGG; hsa:55506; - UCSC; uc001jqm.4; human CTD; 55506; - DisGeNET; 55506; - EuPathDB; HostDB:ENSG00000099284.13; - GeneCards; H2AFY2; - H-InvDB; HIX0008890; - HGNC; HGNC:14453; H2AFY2 HPA; HPA035865; - HPA; HPA041189; - MIM; 616141; gene neXtProt; NX_Q9P0M6; - OpenTargets; ENSG00000099284; - PharmGKB; PA29118; - eggNOG; ENOG410IV3A; Eukaryota eggNOG; KOG1756; Eukaryota eggNOG; KOG2633; Eukaryota eggNOG; COG2110; LUCA eggNOG; COG5262; LUCA GeneTree; ENSGT00940000158120; - HOGENOM; HOG000234653; - HOVERGEN; HBG009342; - InParanoid; Q9P0M6; - KO; K11251; - OMA; MKVEGIV; - OrthoDB; 1504122at2759; - PhylomeDB; Q9P0M6; - TreeFam; TF332276; - ChiTaRS; H2AFY2; human EvolutionaryTrace; Q9P0M6; - GeneWiki; H2AFY2; - GenomeRNAi; 55506; - PRO; PR:Q9P0M6; - Proteomes; UP000005640; Chromosome 10 Bgee; ENSG00000099284; Expressed in 226 organ(s), highest expression level in embryo ExpressionAtlas; Q9P0M6; baseline and differential Genevisible; Q9P0M6; HS GO; GO:0001740; C:Barr body; IDA:MGI GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB GO; GO:0000790; C:nuclear chromatin; IDA:UniProtKB GO; GO:0000784; C:nuclear chromosome, telomeric region; HDA:BHF-UCL GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW GO; GO:0031490; F:chromatin DNA binding; IDA:UniProtKB GO; GO:0003677; F:DNA binding; IBA:GO_Central GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro GO; GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:UniProtKB GO; GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB GO; GO:0007420; P:brain development; IMP:UniProtKB GO; GO:0006325; P:chromatin organization; IBA:GO_Central GO; GO:0007549; P:dosage compensation; IDA:MGI GO; GO:0071169; P:establishment of protein localization to chromatin; IMP:UniProtKB GO; GO:0045814; P:negative regulation of gene expression, epigenetic; IMP:UniProtKB GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB GO; GO:1901837; P:negative regulation of transcription of nucleolar large rRNA by RNA polymerase I; IGI:UniProtKB GO; GO:0006334; P:nucleosome assembly; IEA:InterPro GO; GO:0045618; P:positive regulation of keratinocyte differentiation; IMP:UniProtKB CDD; cd00074; H2A; 1 CDD; cd02904; Macro_H2A_like; 1 Gene3D; 1.10.20.10; -; 1 InterPro; IPR021171; Core_histone_macro-H2A InterPro; IPR009072; Histone-fold InterPro; IPR002119; Histone_H2A InterPro; IPR007125; Histone_H2A/H2B/H3 InterPro; IPR032454; Histone_H2A_C InterPro; IPR002589; Macro_dom InterPro; IPR035796; Macro_H2A Pfam; PF00125; Histone; 1 Pfam; PF16211; Histone_H2A_C; 1 Pfam; PF01661; Macro; 1 PIRSF; PIRSF037942; Core_histone_macro-H2A; 1 PRINTS; PR00620; HISTONEH2A SMART; SM00506; A1pp; 1 SMART; SM00414; H2A; 1 SUPFAM; SSF47113; SSF47113; 1 PROSITE; PS51154; MACRO; 1 1: Evidence at protein level; 3D-structure; Chromatin regulator; Chromosome; Complete proteome; DNA-binding; Isopeptide bond; Nucleosome core; Nucleus; Reference proteome; Ubl conjugation CHAIN 1 372 Core histone macro-H2A.2 /FTId=PRO_0000055320 DOMAIN 2 117 Histone H2A DOMAIN 184 370 Macro. {ECO:0000255|PROSITE- ProRule:PRU00490} COMPBIAS 118 161 Lys-rich CROSSLNK 239 239 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) STRAND 183 191 {ECO:0000244|PDB:6FY5} STRAND 196 202 {ECO:0000244|PDB:6FY5} HELIX 204 209 {ECO:0000244|PDB:6FY5} STRAND 213 219 {ECO:0000244|PDB:6FY5} HELIX 227 252 {ECO:0000244|PDB:6FY5} STRAND 260 264 {ECO:0000244|PDB:6FY5} STRAND 266 277 {ECO:0000244|PDB:6FY5} HELIX 286 302 {ECO:0000244|PDB:6FY5} TURN 303 305 {ECO:0000244|PDB:6FY5} STRAND 307 311 {ECO:0000244|PDB:6FY5} HELIX 323 339 {ECO:0000244|PDB:6FY5} STRAND 348 353 {ECO:0000244|PDB:6FY5} HELIX 356 366 {ECO:0000244|PDB:6FY5} STRAND 369 371 {ECO:0000244|PDB:6FY5} SEQUENCE 372 AA; 40058 MW; C4B35CD156031AF1 CRC64; MSGRSGKKKM SKLSRSARAG VIFPVGRLMR YLKKGTFKYR ISVGAPVYMA AVIEYLAAEI LELAGNAARD NKKARIAPRH ILLAVANDEE LNQLLKGVTI ASGGVLPRIH PELLAKKRGT KGKSETILSP PPEKRGRKAT SGKKGGKKSK AAKPRTSKKS KPKDSDKEGT SNSTSEDGPG DGFTILSSKS LVLGQKLSLT QSDISHIGSM RVEGIVHPTT AEIDLKEDIG KALEKAGGKE FLETVKELRK SQGPLEVAEA AVSQSSGLAA KFVIHCHIPQ WGSDKCEEQL EETIKNCLSA AEDKKLKSVA FPPFPSGRNC FPKQTAAQVT LKAISAHFDD SSASSLKNVY FLLFDSESIG IYVQEMAKLD AK
Research Areas

BACKGROUND

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

REFERENCES

Xu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):2136-2140(2010)
Sporn, J.C., et al. Oncogene 28(38):3423-3428(2009)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Zhang, R., et al. Dev. Cell 8(1):19-30(2005)
Deloukas, P., et al. Nature 429(6990):375-381(2004)

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