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USP21 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - USP21 Antibody (N-term) AP1069a
    All lanes : Anti-hUSP21-M1 at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - USP21 Antibody (N-term) AP1069a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9UK80
Other Accession B2GUX4, Q9QZL6, Q2KJ72
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62656 Da
Additional info
Gene ID 27005
Other Names Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23
Target/Specificity This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human USP21.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP21 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP21
Synonyms USP23
Function Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.
Cellular Location Cytoplasm. Nucleus
Tissue Location Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung
Research Areas
The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog.
Author : Jin J1,Liu J2,3,Chen C1,Liu Z4,Jiang C1,Chu H1,Pan W1,Wang X1,Zhang L5,Li B6,Jiang C4,Ge X7,Xie X2,3,Wang P1,4.
Nat Commun. 2016 Nov 25;7:13594. doi: 10.1038/ncomms13594.
27886188
Deubiquitination and stabilization of IL-33 by USP21.
Author : Tao L1, Chen C2, Song H1, Piccioni M2, Shi G1, Li B2.
Int J Clin Exp Pathol. 2014 Jul 15;7(8):4930-7. eCollection 2014.
25197364
Identification of the E3 deubiquitinase ubiquitin-specific peptidase 21 (USP21) as a positive regulator of the transcription factor GATA3.
Author : Zhang J1, Chen C, Hou X, Gao Y, Lin F, Yang J, Gao Z, Pan L, Tao L, Wen C, Yao Z, Tsun A, Shi G, Li B.
J Biol Chem. 2013 Mar 29;288(13):9373-82. doi: 10.1074/jbc.M112.374744. Epub 2013 Feb 10.
23395819

BACKGROUND

USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.

REFERENCES

Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000).
Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996).
Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).

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