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B3GNT4 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - B3GNT4 Antibody (Center) AP10696c
    B3GNT4 Antaibody (Center) (Cat. #AP10696c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).
  • 1 - B3GNT4 Antibody (Center) AP10696c
    B3GNT4 Antaibody (Center) (Cat. #AP10696c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).
  • 4 - B3GNT4 Antibody (Center) AP10696c
    B3GNT4 Antibody (Center) (Cat. #AP10696c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q9C0J1
Other Accession NP_110392.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42310 Da
Additional info
Gene ID 79369
Other Names N-acetyllactosaminide beta-1, 3-N-acetylglucosaminyltransferase 4, UDP-GlcNAc:betaGal beta-1, 3-N-acetylglucosaminyltransferase 4, BGnT-4, Beta-1, 3-Gn-T4, Beta-1, 3-N-acetylglucosaminyltransferase 4, Beta3Gn-T4, B3GNT4
Target/Specificity This B3GNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-260 amino acids from the Central region of human B3GNT4.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB3GNT4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B3GNT4
Function Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Mainly expressed in brain tissues such as whole brain, hippocampus, amygdala, cerebellum and caudate nucleus. Also expressed in colon, esophagus and kidney
Research Areas

BACKGROUND

This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein. [provided by RefSeq].

REFERENCES

Shiraishi, N., et al. J. Biol. Chem. 276(5):3498-3507(2001)
Amado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999)

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