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CSF2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CSF2 Antibody (Center) AP10690c
    CSF2 Antibody (Center) (Cat. #AP10690c) western blot analysis in HL60 cell line lysates (35ug/lane).This demonstrates the CSF2 antibody detected the CSF2 protein (arrow).
  • 14 - CSF2 Antibody (Center) AP10690c
    CSF2 antibody (Center) (Cat. #AP10690c) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CSF2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - CSF2 Antibody (Center) AP10690c
    CSF2 Antibody (Center) (Cat. #AP10690c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - CSF2 Antibody (Center) AP10690c
    Confocal immunofluorescent analysis of CSF2 Antibody (Center) (Cat#AP10690c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, IF, E
Primary Accession P04141
Other Accession NP_000749.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16295 Da
Additional info
Gene ID 1437
Other Names Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF
Target/Specificity This CSF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-85 amino acids from the Central region of human CSF2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCSF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CSF2
Synonyms GMCSF
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Cellular Location Secreted
Research Areas
Osteoarthritic changes in vervet monkey knees correlate with meniscus degradation and increased matrix metalloproteinase and cytokine secretion.
Author : Stone AV1, Vanderman KS2, Willey JS3, Long DL4, Register TC5, Shively CA6, Stehle JR Jr7, Loeser RF8, Ferguson CM9.
Osteoarthritis Cartilage. 2015 May 29. pii: S1063-4584(15)01177-2. doi: 10.1016/j.joca.2015.05.020. [Epub ahead of print]
26033163

BACKGROUND

CSF2 is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq].

REFERENCES

Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Beckers, M.M., et al. Eur. J. Intern. Med. 21(4):289-292(2010)
Wang, Y., et al. Diabet. Med. 27(4):376-383(2010)
Katano, M., et al. Arthritis Res. Ther. 11 (1), R3 (2009) :

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