|Application ||WB, E|
|Other Names||Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2|
|Target/Specificity||This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-151 amino acids from the N-terminal region of human MBD2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MBD2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.|
|Cellular Location||Nucleus. Note=Nuclear, in discrete foci Detected at replication foci in late S phase|
|Tissue Location||Highly expressed in brain, heart, kidney, stomach, testis and placenta.|
Provided below are standard protocols that you may find useful for product applications.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Zhu, Y., et al., Cancer 100(9):1853-1858 (2004).
Ghoshal, K., et al., J. Biol. Chem. 279(8):6783-6793 (2004).
Fujita, H., et al., Mol. Cell. Biol. 23(8):2645-2657 (2003).
Patra, S.K., et al., Biochem. Biophys. Res. Commun. 302(4):759-766 (2003).
Lembo, F., et al., Mol. Cell. Biol. 23(5):1656-1665 (2003).