注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   神经科学   >   CD11b Antibody (N-term)   

CD11b Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - CD11b Antibody (N-term) AP10668a
    AP10668a staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - CD11b Antibody (N-term) AP10668a
    AP10668a staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - CD11b Antibody (N-term) AP10668a
    AP10668a staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - CD11b Antibody (N-term) AP10668a
    Anti-CD11b Antibody (N-term) at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CD11b Antibody (N-term) AP10668a
    Anti-CD11b Antibody (N-term) at 1:500 dilution + human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - CD11b Antibody (N-term) AP10668a
    CD11b Antibody (N-term) (Cat. #AP10668a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 1 - CD11b Antibody (N-term) AP10668a
    CD11b Antibody (N-term) (Cat. #AP10668a) western blot analysis in A549,K562 cell line lysates (35ug/lane).This demonstrates the CD11b antibody detected the CD11b protein (arrow).
  • 产品详情
  • 文献引用 : 1
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, IHC-P, E
Primary Accession P11215
Other Accession NP_000623
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 127179 Da
Additional info
Gene ID 3684
Other Names Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A
Target/Specificity This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
Dilution IHC-P~~1:25
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD11b Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ITGAM
Synonyms CD11B, CR3A
Function Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177- PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein
Tissue Location Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).
Research Areas
Repetitive myocardial ischemia promotes coronary growth in the adult mammalian heart.
Author : Lavine KJ1, Kovacs A, Weinheimer C, Mann DL.
J Am Heart Assoc. 2013 Sep 30;2(5):e000343. doi: 10.1161/JAHA.113.000343.
24080909

BACKGROUND

CD11b is the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

REFERENCES

Gjelstrup, L.C., et al. J. Immunol. 185(7):4154-4168(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Warchol, T., et al. DNA Cell Biol. (2010) In press :
Fan, Y., et al. J Eur Acad Dermatol Venereol (2010) In press :
Pliyev, B.K., et al. Biochem. Biophys. Res. Commun. 397(2):277-282(2010)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价