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CD34 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CD34 Antibody (Center) AP10666c
    CD34 Antibody (Center) (Cat. #AP10666c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CD34 antibody detected the CD34 protein (arrow).
  • 4 - CD34 Antibody (Center) AP10666c
    CD34 Antibody (Center) (Cat. #AP10666c) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P28906
Other Accession NP_001020280
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40716 Da
Additional info
Gene ID 947
Other Names Hematopoietic progenitor cell antigen CD34, CD34, CD34
Target/Specificity This CD34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the Central region of human CD34.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD34 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD34
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues
Research Areas

BACKGROUND

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.

REFERENCES

Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010)
McCalmont, T.H., et al. J. Cutan. Pathol. 37 (9), 923 (2010) :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Cho, S.Y., et al. Cancer Res. 70(8):3402-3410(2010)
Gluhovschi, C., et al. Folia Histochem. Cytobiol. 48(2):230-236(2010)

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