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CES2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - CES2 Antibody (Center) AP10661c
    Overlay histogram showing MCF-7 cells stained with AP10661c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10661c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 4 - CES2 Antibody (Center) AP10661c
    Overlay histogram showing U-87 MG cells stained with AP10661c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10661c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - CES2 Antibody (Center) AP10661c
    All lanes : Anti-CES2 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CES2 Antibody (Center) AP10661c
    All lanes : Anti-CES2 Antibody (Center) at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CES2 Antibody (Center) AP10661c
    CES2 Antibody (Center) (Cat. #AP10661c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CES2 antibody detected the CES2 protein (arrow).
  • 14 - CES2 Antibody (Center) AP10661c
    CES2 antibody (Center) (Cat. #AP10661c) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CES2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession O00748
Other Accession NP_003860
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61807 Da
Additional info
Gene ID 8824
Other Names Cocaine esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate deacetylase 2, CES2, ICE
Target/Specificity This CES2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 340-369 amino acids from the Central region of human CES2.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCES2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CES2
Synonyms ICE
Function Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:9169443). Shows high catalytic efficiency for hydrolysis of cocaine, 4- methylumbelliferyl acetate, heroin and 6-monoacetylmorphine (PubMed:9169443). Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol (PubMed:28677105).
Cellular Location Endoplasmic reticulum lumen
Tissue Location Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.
Research Areas
Fluoxetine reduces CES1, CES2, and CYP3A4 expression through decreasing PXR and increasing DEC1 in HepG2 cells.
Author : Shang W1, Liu J, Chen R, Ning R, Xiong J, Liu W, Mao Z, Hu G, Yang J.
Xenobiotica. 2015 Sep 4:1-13. [Epub ahead of print]
26340669
Decreased carboxylesterases expression and hydrolytic activity in type 2 diabetic mice through Akt/mTOR/HIF-1α/Stra13 pathway.
Author : Chen R1, Wang Y, Ning R, Hu J, Liu W, Xiong J, Wu L, Liu J, Hu G, Yang J.
Xenobiotica. 2015;45(9):782-93. doi: 10.3109/00498254.2015.1020353. Epub 2015 Jul 27.
25801056

BACKGROUND

CES2 is a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance.

REFERENCES

Holmes, R.S., et al. Mamm. Genome 21 (9-10), 427-441 (2010) : Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Howard, T.D., et al. Environ. Health Perspect. 118(10):1395-1399(2010) Hatfield, M.J., et al. Br. J. Pharmacol. 160(8):1916-1928(2010) Holmes, R.S., et al. Genetica 138(7):695-708(2010)

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