- 文献引用 : 2
|Application ||WB, IHC-P, FC, E|
|Calculated MW||61807 Da|
|Other Names||Cocaine esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate deacetylase 2, CES2, ICE|
|Target/Specificity||This CES2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 340-369 amino acids from the Central region of human CES2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CES2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:9169443). Shows high catalytic efficiency for hydrolysis of cocaine, 4- methylumbelliferyl acetate, heroin and 6-monoacetylmorphine (PubMed:9169443). Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol (PubMed:28677105).|
|Cellular Location||Endoplasmic reticulum lumen|
|Tissue Location||Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.|
Author : Shang W1, Liu J, Chen R, Ning R, Xiong J, Liu W, Mao Z, Hu G, Yang J.
Xenobiotica. 2015 Sep 4:1-13. [Epub ahead of print]
Author : Chen R1, Wang Y, Ning R, Hu J, Liu W, Xiong J, Wu L, Liu J, Hu G, Yang J.
Xenobiotica. 2015;45(9):782-93. doi: 10.3109/00498254.2015.1020353. Epub 2015 Jul 27.
Provided below are standard protocols that you may find useful for product applications.
CES2 is a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance.
Holmes, R.S., et al. Mamm. Genome 21 (9-10), 427-441 (2010) : Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Howard, T.D., et al. Environ. Health Perspect. 118(10):1395-1399(2010) Hatfield, M.J., et al. Br. J. Pharmacol. 160(8):1916-1928(2010) Holmes, R.S., et al. Genetica 138(7):695-708(2010)