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UBC9 (UBE2I) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - UBC9 (UBE2I) Antibody (N-term) AP1064a
    Western blot analysis of anti-UBE2I Antibody (N-term) (Cat.#AP1064a) in A2058 cell line lysates (35ug/lane). UBE2I(arrow) was detected using the purified Pab.
  • 14 - UBC9 (UBE2I) Antibody (N-term) AP1064a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - UBC9 (UBE2I) Antibody (N-term) AP1064a
    Western blot analysis of UBE2I (arrow) using rabbit polyclonal UBE2I Antibody (S7) (Cat.#AP1064a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P63279
Other Accession P63282, P63281, P63280, P63283, Q9DDJ0, Q9W6H5
Reactivity Human
Predicted Zebrafish, Chicken, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18007 Da
Additional info
Gene ID 7329
Other Names SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9
Target/Specificity This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBC9 (UBE2I) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2I
Synonyms UBC9, UBCE9
Function Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.
Cellular Location Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).
Tissue Location Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Research Areas
UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum.
Author : Lee D1, Kraus A1, Prins D1, Groenendyk J1, Aubry I2, Liu WX1, Li HD1, Julien O1, Touret N1, Sykes BD1, Tremblay ML2, Michalak M3<
J Biol Chem. 2015 Jan 13. pii: jbc.M114.635474. [Epub ahead of print]
25586181
MDA5 is SUMOylated by PIAS2β in the upregulation of type I interferon signaling.
Author : Fu J, Xiong Y, Xu Y, Cheng G, Tang H.
Mol Immunol. 2011 Jan;48(4):415-22. doi: 10.1016/j.molimm.2010.09.003. Epub 2010 Dec 14.
21156324

BACKGROUND

UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.

REFERENCES

Biochem Biophys Res Commun. 2002 Aug 30;296(4):870-6.
Genomics. 1996 Oct 15;37(2):183-6.
Cytogenet Cell Genet. 1996;75(4):222-3.
Cytogenet Cell Genet. 1996;72(1):86-9.

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