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UBE1C Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - UBE1C Antibody (C-term) AP1063b
    The anti-UBE1 C-term Pab (Cat. #AP1063b) is used in Western blot to detect UBE1 in mouse brain tissue lysate.
  • 14 - UBE1C Antibody (C-term) AP1063b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - UBE1C Antibody (C-term) AP1063b
    UBE1C Antibody (S430) (Cat. #AP1063b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).
  • 1 - UBE1C Antibody (C-term) AP1063b
    Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C Antibody (S430) (Cat. #AP1063b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q8TBC4
Other Accession Q99MI7, Q8C878, Q7ZVX6
Reactivity Human, Mouse
Predicted Zebrafish, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51852 Da
Additional info
Gene ID 9039
Other Names NEDD8-activating enzyme E1 catalytic subunit, 632-, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, UBA3, UBE1C
Target/Specificity This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 amino acids from the C-terminal region of human UBE1C.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE1C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBA3
Synonyms UBE1C
Function Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
Tissue Location Ubiquitously expressed.
Research Areas

BACKGROUND

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

REFERENCES

Bohnsack, R.N., et al., J. Biol. Chem. 278(29):26823-26830 (2003).
Walden, H., et al., Nature 422(6929):330-334 (2003).
Gong, L., et al., J. Biol. Chem. 274(17):12036-12042 (1999).
Gubin, A.N., et al., Genomics 59(2):168-177 (1999).
Osaka, F., et al., Genes Dev. 12(15):2263-2268 (1998).

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